Advertisement

Conservation Genetics

, Volume 10, Issue 3, pp 507–509 | Cite as

Isolation and characterization of microsatellite loci in Shortia rotundifolia (Diapensiaceae), an endangered relict plant on the Ryukyu Islands and Taiwan

  • Tomomi Dan
  • Yuki Mitsui
  • Hajime Ikeda
  • Yuji Isagi
  • Hiroaki Setoguchi
Technical Note

Abstract

Ten microsatellite loci were isolated and characterized for Shortia rotundifolia, a relict temperate plant on the subtropical Ryukyu Islands and Taiwan. The number of alleles ranged from 2 to 21. The expected (H E) and observed (H O) heterozygosities were 0.027–0.877 and 0.027–0.622, respectively, from 74 individuals on Iriomotejima Island of Ryukyus. One locus exhibited significantly fewer heterozygotes than expected under Hardy–Weinberg equilibrium (< 0.05). These primers amplifying microsatellites in this species may provide a useful tool for population genetics to establish conservation strategy.

Keywords

Conservation genetics Heterozygosity Microsatellite Ryukyu Islands Shortia rotundifolia 

References

  1. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from plant tissue. Focus 12:13–15Google Scholar
  2. Environmental Agency of Japan (2000) Threatened wildlife of Japan, Red Data Book, 2nd edn, vol 8 (vascular plants). Japan Wildlife Research Center, Tokyo (in Japanese)Google Scholar
  3. Goudet J (1995) Fstat version 1.2: a computer program to calculate Fstatistics. J Heredity 86:485–486Google Scholar
  4. Kimura M (1996) Quaternary paleogeography of the Ryukyu Arc. J Geog 105:259–285 (in Japanese with English abstract)Google Scholar
  5. Kimura M (2000) Paleogeography of the Ryukyu Islands. Tropics 10:5–24CrossRefGoogle Scholar
  6. Kizaki K, Oshiro I (1977) Paleogeography of the Ryukyu Islands. Marine Sci Mon 9:542–549 (in Japanese with English abstract)Google Scholar
  7. Kizaki K, Oshiro I (1980) The origin of the Ryukyu Islands. In: Kizaki K (ed) Natural history of the Ryukyus. Tsukiji-Shokan, Tokyo, pp 8–37 (in Japanese)Google Scholar
  8. Lian CL, Hougetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-supressin PCR technique. Mol Ecol Notes 2:211–213CrossRefGoogle Scholar
  9. Lian CL, Wadud MA, Geng Q, Shimatani K, Hogetsu T (2006) An improved technique for isolating codominant microsatellite markers. J Plant Res 119:415–417PubMedCrossRefGoogle Scholar
  10. Setoguchi H, Ohba H (1995) Phylogenetic relationships in Crossostylis inferred from restriction site variation of chloroplast DNA. J Plant Res 108:87–92CrossRefGoogle Scholar
  11. Ujiie H (1986) The Sea-Bed of the Ryukyu Islands Arc. In: Sinseitosho-shuppan, NahaGoogle Scholar
  12. Ujiie H (1996) Geological history of the Ryukyu Island Arc. In: Ujiie H (ed) Nature of Okinawa, geomorphology and geology. Hirugisha, Naha, pp 251–255 (in Japanese)Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Tomomi Dan
    • 1
  • Yuki Mitsui
    • 1
  • Hajime Ikeda
    • 1
  • Yuji Isagi
    • 2
  • Hiroaki Setoguchi
    • 1
  1. 1.Graduate School of Human and Environmental StudiesKyoto University, Yoshida Nihonmatsu-choKyotoJapan
  2. 2.Laboratory of Forest Biology, Division of Forest and Biomaterials ScienceGraduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-choKyotoJapan

Personalised recommendations