Development of thirteen microsatellite loci for Impatiens lateristachys (Balsaminaceae)
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Impatiens lateristachys is a local endemic species of Emei Mountain in southwestern China. In order to study the speciation mode of this species and its closely relatives, it is important to characterize their genetic diversity and differentiation. Here, thirteen polymorphic microsatellite markers have been developed for I. lateristachys. AC/TG microsatellite was enriched by combining biotin capture method. The average allele number of these markers was 3.1, ranging from 2 to 6. The observed (H O) and expected (H E) heterozygosities were 0.15−0.55 and 0.21–0.70, respectively. All the 13 markers could be applied in the other four congeneric species.
KeywordsGenetic diversity Impatiens lateristachys Microsatellite markers Speciation mode
This work was financially supported by the “Talented People Project of the Sun Yat-Sen University” granted to X.-J. Ge, and by Swiss National Science Foundation (FN 3100AO-102165) granted to Y.-M. Yuan.
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