Microsatellite markers developed for a Swedish population of sand lizard (Lacerta agilis)
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Populations of sand lizards (Lacerta agilis) are declining throughout its north-western range. Here we characterize fifteen new microsatellite markers developed specifically for parentage analysis in a small Swedish population of sand lizards. These loci were screened in the Asketunnan population and a much larger and genetically diverse Hungarian population, with heterozygosities ranging from (0.217–0.875) and (0.400–0.974), respectively. All loci were in Hardy-Weinberg Equilibrium in the Swedish population but eight loci had significant heterozygote deficiencies in the Hungarian population. Two loci were significantly linked in both populations. These microsatellite loci are likely to be applicable in research on other sand lizard populations throughout Europe.
KeywordsDNA markers Microsatellite Simple tandom repeat Paternity Lacertid
We would like to T. Madsen, B. Ujvari, and E. Wapstra for the collection of samples. This research was funded by an Australian Research Council Discovery Grant to MO, and a New South Wales BioFirst Award from the New South Wales Office of Science & Medical Research awarded to MO.
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