Conservation Genetics

, Volume 9, Issue 3, pp 715–717 | Cite as

Microsatellite markers developed for a Swedish population of sand lizard (Lacerta agilis)

  • Tonia S. Schwartz
  • Mats Olsson
Technical Note


Populations of sand lizards (Lacerta agilis) are declining throughout its north-western range. Here we characterize fifteen new microsatellite markers developed specifically for parentage analysis in a small Swedish population of sand lizards. These loci were screened in the Asketunnan population and a much larger and genetically diverse Hungarian population, with heterozygosities ranging from (0.217–0.875) and (0.400–0.974), respectively. All loci were in Hardy-Weinberg Equilibrium in the Swedish population but eight loci had significant heterozygote deficiencies in the Hungarian population. Two loci were significantly linked in both populations. These microsatellite loci are likely to be applicable in research on other sand lizard populations throughout Europe.


DNA markers Microsatellite Simple tandom repeat Paternity Lacertid 



We would like to T. Madsen, B. Ujvari, and E. Wapstra for the collection of samples. This research was funded by an Australian Research Council Discovery Grant to MO, and a New South Wales BioFirst Award from the New South Wales Office of Science & Medical Research awarded to MO.


  1. Bohme MU, Berendonk TU, Schlegel M (2005) Isolation of new microsatellite loci from the Green Lizard (Lacerta viridis viridis). Mol Ecol Notes 5:45–47CrossRefGoogle Scholar
  2. Boudjemadi K, Martin O, Simon JC, Estoup A (1999) Development and cross-species comparison of microsatellite markers in two lizard species, Lacerta vivipara and Podarcis muralis. Mol Ecol 8:518–520PubMedGoogle Scholar
  3. Corbett K (1989) Conservation of European reptiles & amphibians. Christopher Helm, LondonGoogle Scholar
  4. Excoffier L, Lavel G, Schneider S (2005) Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50Google Scholar
  5. Gautschi B, Tenzer I, Müller JP, Schmid B (2000a) Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and cross-amplificaion in three Old World vulture species. Mol Ecol 9:2193–2195CrossRefGoogle Scholar
  6. Gautschi B, Widmer A, Koella J (2000b) Isolation and characterization of microsatellite loci in the Dice Snake (Natrix tessellata). Mol Ecol 9:2191–2193Google Scholar
  7. Gullberg A, Olsson M, Tegelstrom H (1999) Evolution in populations of Swedish sand lizards: genetic differentiation and loss of variability revealed by multilocus DNA fingerprinting. J Evol Biol 12:17–26CrossRefGoogle Scholar
  8. Gullberg A, Tegelstrom H, Olsson M (1997) Microsatellites in the sand lizard (Lacerta agilis): Description, variation, inheritance, and applicability. Biochem Genet 35:281–295PubMedCrossRefGoogle Scholar
  9. Madsen T, Olsson M, Wittzell H et al. (2000) Population size and genetic diversity in sand lizards (Lacerta agilis) and adders (Vipera berus). Biol Conserv 94:257–262CrossRefGoogle Scholar
  10. Olsson M, Gullberg A, Tegelstrom H (1996) Malformed offspring, sibling matings, and selection against inbreeding in the sand lizard (Lacerta agilis). J Evol Biol 9:229–242CrossRefGoogle Scholar
  11. Raymond M, Rousset F (1995) Population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  12. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  13. Tenzer I, degli Ivanissevich S, Morgante M, Gessler C (1999) Identification of microsatellite markers and their application to population genetics of Venturia inaequalis. Phytopathylolgy 89:748–753CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Institute for Conservation Biology, School of Biological SciencesUniversity of WollongongWollongongAustralia

Personalised recommendations