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Identification and characterization of microsatellites for striped bass from repeat-enriched libraries

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Abstract

Striped bass (Morone saxatilis) is economically important in the US due to its value as an aquaculture species and in supporting commercial and recreational fisheries, especially those off the Atlantic coast and in the Gulf of Mexico. Modern strategies for managing fishery populations and aquaculture broodstocks employ the use of molecular genetic markers to identify individuals, assign parentage, and characterize population genetic structure and levels of inbreeding and migration. As part of a collaborative effort to utilize molecular genetic technologies in striped bass breeding programs we generated microsatellite markers for use in population genetic studies, broodstock selection and management strategies, and the construction of a genetic map. We developed 345 new microsatellite markers for striped bass, a subset (n=71) of which was characterized by genotyping samples from two striped bass broodstock populations to evaluate marker polymorphism, percent heterozygosity, Hardy–Weinberg equilibrium (HWE), linkage disequilibrium (LD) and utility for population genetic studies.

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Acknowledgments

The authors would like to acknowledge Dr Curry Woods from UMD and Dr Andy S. McGinty from NCSU for contributing broodstock samples and Roseanna Athey, Kristy Anderson, Michelle Fincham, and James Field for their technical assistance. This work was supported, in part, by a University of North Carolina Office of the President Genomic Sciences Initiative grant (RA02-06) to CVS.

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Correspondence to Caird Rexroad.

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Rexroad, C., Vallejo, R., Coulibaly, I. et al. Identification and characterization of microsatellites for striped bass from repeat-enriched libraries. Conserv Genet 7, 971–982 (2006). https://doi.org/10.1007/s10592-006-9122-0

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  • DOI: https://doi.org/10.1007/s10592-006-9122-0

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