Clinical & Experimental Metastasis

, Volume 29, Issue 8, pp 971–979 | Cite as

Differential effects of vitamin D treatment on inflammatory and non-inflammatory breast cancer cell lines

  • Rebecca L. Hillyer
  • Padma Sirinvasin
  • Madhura Joglekar
  • Robert A. Sikes
  • Kenneth L. van Golen
  • Anja NoheEmail author
Research Paper


Vitamin D is a known regulator of breast cancer cell proliferation, apoptosis, migration, invasion and differentiation in vitro. Recent studies have suggested a preventative role for vitamin D in breast cancer development and suggested a possible therapeutic application of vitamin D for patients with various forms of breast cancer. Inflammatory breast cancer (IBC) is a highly aggressive and phenotypically unique form of breast cancer that has a very poor prognosis. IBC invades the dermal lymphatics of the breast as tumor emboli early in the course of the disease. Because of the invasive nature of IBC, novel therapeutics are needed desperately. In the current study we examined the effect of the active form of vitamin D, calcitriol, treatment on the aggressive IBC phenotype. Herein we demonstrate that although the vitamin D receptor (VDR) is present in both IBC and non-IBC cell lines, the effect of vitamin D treatment is significant only on the IBC cells. SUM149 IBC cells showed increased protein concentration in response to 24 h of calcitriol exposure; likely mediated by an increase in protein synthesis as opposed to increased cellular proliferation. In addition, treatment with 100 nM calcitriol showed a significant decrease in SUM149 migration (67.8 % decrease, P = 0.030), invasion (43.9 % decrease, P = 0.015), and tumor spheroid size (69.4 % decrease, P = 0.018) compared to nontreated control groups. Finally, calcitriol treatment of SUM149 cells led to significantly fewer IBC experimental metastases as compared to control. Our study demonstrates that calcitriol treatment of SUM149 affected several of the processes important for IBC metastasis but had little effect on MDA-MB-231 cells. Therefore, calcitriol treatment may have the potential to decrease the rate and incidence of metastasis in IBC patients.


Calcitriol Inflammatory breast cancer Invasion MDA-MB-231 Migration SUM149 Tumor emboli Vitamin D Vitamin D receptor 



Vitamin D receptor


Vitamin D response elements


Inflammatory breast cancer


Fetal bovine serum


Phosphate buffered saline



We would like to thank Dr. Kirk Czymmek and the Delaware Biotechnology Institute for their assistance with imaging and computer analysis. We would also like to thank Vimal Gangadharan and Heather Unger for their help with experimental procedures We thank Jomnarong Lertsuwan and Adam Aguiar, for their help with RT-PCR and scratch assay methods.


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Rebecca L. Hillyer
    • 1
  • Padma Sirinvasin
    • 1
  • Madhura Joglekar
    • 1
  • Robert A. Sikes
    • 1
    • 2
  • Kenneth L. van Golen
    • 1
    • 2
  • Anja Nohe
    • 1
    • 2
    Email author
  1. 1.The Department of Biological ScienceThe University of DelawareNewarkUSA
  2. 2.The Center for Translational Cancer ResearchThe University of DelawareNewarkUSA

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