Clinical & Experimental Metastasis

, Volume 28, Issue 5, pp 413–425 | Cite as

Identification of annexin A1 as a proinvasive and prognostic factor for lung adenocarcinoma

Research Paper


Metastasis is the most common cause of death in lung cancer patients and is a major obstacle to the successful treatment. To discover novel metastasis-related proteins in lung adenorcinoma (AdC), quantitative proteomic analysis was performed between primary lung AdC tissues with (LNM AdC) and without lymph node metastasis (non-LNM AdC). In this study, annexin A1 was identified to be significantly up-regulated in LNM AdC compared with non-LNM AdC. Immunohistochemistry showed that annexin A1 over-expression was frequently observed in LNM AdCs and matched lymph node metastases compared with non-LNM AdCs. Annexin A1 over-expression was significantly associated with advanced clinical stage (P < 0.05) and lymph node metastasis (P < 0.05) and increased relapse rate (P < 0.05) and decreased overall survival (P < 0.05) in lung AdCs. Cox regression analysis indicated annexin A1 over-expression was an independent prognostic factor. Furthermore, suppression of annexin A1 expression by siRNA interference significantly inhibited the invasion ability of lung adenocarcinoma cell A549 in vitro. In conclusion, annexin A1 expression correlated with tumor stage, lymph node metastasis, relapse, and patient survival. Annexin A1 is proposed to function importantly in the progression of lung AdC.


Annexin A1 Lung adenocarcinoma Proteomics Invasion Prognostic factor 


Lung AdC

Lung adenocarcinoma


Lymph node metastasis


Lung adenocarcinoma without lymph node metastasis


Lung adenocarcinoma with lymph node metastasis


Two-dimensional difference gel electrophoresis

Positive LN

Positive lymph node


Hazard ratio


Confidence interval



This work was supported by grants from National Key Basic Research Program of China (2001CB510207), the Fundamental Research Funds for the Central Universities (2010121104) and Outstanding Scholars of New Era from Ministry of Education of China (2002-48).

Conflicts of interest

No conflicts of interest are declared.


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of Basic Medical Sciences, Medical CollegeXiamen UniversityXiamenChina
  2. 2.Key Laboratory of Cancer Proteomics of Ministry of HealthCentral South UniversityChangshaChina

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