Chromosome Research

, Volume 21, Issue 8, pp 753–763 | Cite as

Dissociation of the X chromosome from the synaptonemal complex in the XY body of the rodent Galea musteloides

  • Roberta B. Sciurano
  • I. Mónica Rahn
  • Juan C. Cavicchia
  • Alberto J. Solari


The XY body from spermatocytes of the rodent Galea musteloides shows progressive changes of the synaptonemal complex (SC) axes and the X-chromatin during pachynema. There is a gross thickening of the X-axis and the formation of a large X chromosome loop at mid and late pachytene stages. The SC proteins synaptonemal complex protein 3 (SYCP3), synaptonemal complex protein 1, and synaptonemal complex central element protein 3 and the proteins breast cancer 1, MutL homolog 1 (MLH1), and radiation-repair 51 (related to meiotic processes), the cohesin structural maintenance of chromosome 3, the centromeric protein (with CREST antibody), and the silenced chromatin (with phosphorylated (139ph) H2A histone family, member X (γ-H2AX) antibody) were analyzed in this XY body. The thick X-axis, including the interstitial loop, becomes formed by four to six laminae showing a cross-striation with a periodicity of about 20 nm. The whole length of the gross X-axis shows no significant changes during pachynema, but the interstitial chromatin of the X chromosome and the X centromere are included in the large loop, and it becomes separated from the SC. A conventional SC formed by the Y-axis, a central region and a thin lateral element originally corresponding to the X-axis, remains undisturbed up to the end of pachynema. A single MLH1 focus develops either at the distal or the proximal region of the loop end attached to the conventional SC. The chromatin surrounding the thickened axis is labeled with γ-H2AX. It is shown that most of the SYCP3 protein associated with the X chromosome loop is not involved in the SC maintenance, but it is located with the cohesin axis separated from the SC proper.


XY body Synaptonemal complexes SYCP3 MLH1 MSCI γ-H2AX 



Ataxia telangiectasia and Rad3 related


Breast cancer 1


Central region


Calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasia


Deoxyribonucleic acid


Electron microscopy


Indirect immunofluorescence


Lateral element


MutL homolog 1


Meiotic sex chromosome inactivation


Meiotic silencing of unsynapsed chromatin


Pseudoautosomal region


Phosphate-buffered saline


Phosphotungstic acid


Radiation-repair 51


Synaptonemal complex


Structural maintenance of chromosome 3


Synaptonemal complex protein 1


Synaptonemal complex protein 3


Synaptonemal complex central element protein 3


Phosphorylated (Ser139) histone 2, A.X



The able help of Lic C. Deparci is sincerely thanked. We are also very grateful to Prof. M. Alsheimer (University of Würzburg, Germany) for the generous gift of SYCE3 antibody and P.J. Moens† and B. Spyropoulus (York University, Canada) for SYCP1 antibody. We specially thank Prof. Dr. A. Brusco and Eng. Lisandro Anton from LANAIS' service (CONICET). This work was supported by grants from UBACYT 20020100100030 (AJS) and PICT-2010-2718 (RBS).


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Roberta B. Sciurano
    • 1
  • I. Mónica Rahn
    • 1
  • Juan C. Cavicchia
    • 2
  • Alberto J. Solari
    • 1
    • 3
  1. 1.2da. UA de Biología Celular, Histología, Embriología y Genética, Facultad de MedicinaUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.IHEM, CCT-CONICETMendozaArgentina
  3. 3.Facultad de MedicinaUniversidad de Buenos AiresBuenos AiresArgentina

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