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Chromosome Research

, Volume 21, Issue 2, pp 121–136 | Cite as

High chromosome variability and the presence of multivalent associations in buthid scorpions

  • Viviane Fagundes Mattos
  • Doralice Maria Cella
  • Leonardo Sousa Carvalho
  • Denise Maria Candido
  • Marielle Cristina Schneider
Article

Abstract

In this study, we investigated the mitotic and meiotic chromosomes of 11 Buthidae scorpion species, belonging to three genera (Ananteris, Rhopalurus and Tityus), to obtain detailed knowledge regarding the mechanisms underlying the intraspecific and/or interspecific diversity of chromosome number and the origin of the complex chromosome associations observed during meiosis. The chromosomes of all species did not exhibit a localised centromere region and presented synaptic and achiasmatic behaviour during meiosis I. Spermatogonial and/or oogonial metaphase cells of these buthids showed diploid numbers range from 2n = 6 to 2n = 28. In most species, multivalent chromosome associations were observed in pachytene and postpachytene nuclei. Moreover, intraspecific variability associated with the presence or absence of chromosome chains and the number of chromosomes in the complex meiotic configurations was observed in some species of these three genera. Silver-impregnated cells revealed that the number and location of nucleolar organiser regions (NORs) remained unchanged despite extensive chromosome variation; notably, two NORs located on the terminal or subterminal chromosome regions were commonly observed for all species. C-banded and fluorochrome-stained cells showed that species with conspicuous blocks of heterochromatin exhibited the lowest rate of chromosomal rearrangement. Based on the investigation of mitotic and meiotic cells, we determined that the intraspecific variability occurred as a consequence of fission/fusion-type chromosomal rearrangements in Ananteris and Tityus species and reciprocal translocation in Rhopalurus species. Furthermore, we verified that individuals presenting the same diploid number differ in structural chromosome organisation, giving rise to intraspecific differences of chromosome association in meiotic cells (bivalent-like elements or chromosome chains).

Keywords

chromosome rearrangements evolution heterozygosity holocentric meiosis nucleolar organiser region 

Abbreviations

C

Chromosome chain

CV

Coefficient of variation

DSL

Diploid set length

NOR

Nucleolar organiser region

II

“Bivalent” or bivalent-like element

CIII

Chain of three chromosomes

CIV

Chain of four chromosomes

CVI

Chain of six chromosomes

CVIII

Chain of eight chromosomes

CX

Chain of 10 chromosomes

CXXVIII

Chain of 28 chromosomes

CMA3

Chromomycin A3

DAPI

4’-6-diamidino-2-phenylindole

Notes

Acknowledgments

The authors would like to thank Dr. Ricardo Pinto-da-Rocha and MSc Sabrina Outeda-Jorge from the Universidade de São Paulo, Dr. Douglas Araujo from the Universidade Estadual de Mato Grosso do Sul and Centro de Controle de Zoonoses from Ituiutaba, state of Minas Gerais, Brazil, for collecting some of the Buthidae specimens. This research was supported through funding from the Fundação de Amparo a Pesquisa do Estado de São Paulo, FAPESP (2010/14226-2, 2011/21643-1) and Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq (478630/2010-7). This work was also part of the “Programa de Pesquisas em Biodiversidade do Semiárido—PPBio Semiárido” (CNPq 558317/2009-0). Collecting permits were granted by the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) and the Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) (25471-1, 25472-1 and 15157-1).

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Viviane Fagundes Mattos
    • 1
  • Doralice Maria Cella
    • 1
  • Leonardo Sousa Carvalho
    • 2
  • Denise Maria Candido
    • 3
  • Marielle Cristina Schneider
    • 4
  1. 1.Instituto de Biociências, Departamento de BiologiaUniversidade Estadual Paulista (UNESP)Rio ClaroBrazil
  2. 2.Universidade Federal do Piauí (UFPI)FlorianoBrazil
  3. 3.Instituto ButantanLaboratório Especial de Coleções ZoológicasSão PauloBrazil
  4. 4.Departamento de Ciências BiológicasUniversidade Federal de São Paulo (UNIFESP)DiademaBrazil

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