Functional centromeres in Astragalus sinicus include a compact centromere-specific histone H3 and a 20-bp tandem repeat
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The centromere plays an essential role for proper chromosome segregation during cell division and usually harbors long arrays of tandem repeated satellite DNA sequences. Although this function is conserved among eukaryotes, the sequences of centromeric DNA repeats are variable. Most of our understanding of functional centromeres, which are defined by localization of a centromere-specific histone H3 (CENH3) protein, comes from model organisms. The components of the functional centromere in legumes are poorly known. The genus Astragalus is a member of the legumes and bears the largest numbers of species among angiosperms. Therefore, we studied the components of centromeres in Astragalus sinicus. We identified the CenH3 homolog of A. sinicus, AsCenH3 that is the most compact in size among higher eukaryotes. A CENH3-based assay revealed the functional centromeric DNA sequences from A. sinicus, called CentAs. The CentAs repeat is localized in A. sinicus centromeres, and comprises an AT-rich tandem repeat with a monomer size of 20 nucleotides.
KeywordsLeguminosae Fabaceae Chinese milk vetch chromatin immunoprecipitation centromere tandem repeat
Astragalus sinicus centromere-specific histone H3
Centromere-specific histone H3
Centromeric DNA sequence of Astragalus sinicus
Fluorescence in situ hybridization
Glycine max centromere-specific histone H3
Polymerase chain reaction
Rapid amplification of cDNA ends
This work was supported by the Fellowship Program of the Japan Society for the Promotion of Science (JSPS) to ALT and KN, and partly by the Turkish Higher Education Council and Harran University, Turkey to ALT.
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