Domain-wide regulation of DNA replication timing during mammalian development
- 762 Downloads
Studies of replication timing provide a handle into previously impenetrable higher-order levels of chromosome organization and their plasticity during development. Although mechanisms regulating replication timing are not clear, novel genome-wide studies provide a thorough survey of the extent to which replication timing is regulated during most of the early cell fate transitions in mammals, revealing coordinated changes of a defined set of 400–800 kb chromosomal segments that involve at least half the genome. Furthermore, changes in replication time are linked to changes in sub-nuclear organization and domain-wide transcriptional potential, and tissue-specific replication timing profiles are conserved from mouse to human, suggesting that the program has developmental significance. Hence, these studies have provided a solid foundation for linking megabase level chromosome structure to function, and suggest a central role for replication in domain-level genome organization.
KeywordsReplication Timing Mammalian Development Cell Differentiation Chromosome Domains Nuclear Organization Chromatin Structure Sub-nuclear Positioning Transcriptional Competence
embryonic stem cell
inactive X chromosome
induced pluripotent stem cell
timing decision point
origin decision point
heterochromatin protein 1
- HMG I/Y
high mobility group proteins I and Y
locus control region
long interspersed nuclear element 1
During development, cell division and differentiation ultimately convert a single totipotent cell into an organized conglomeration of tissues with various specialized functions and features. Along the way, cells are induced to progress down particular paths of differentiation and undergo changes that decrease their responsiveness, or competence, to cues from other cell types (Grimm and Gurdon 2002; Steinbach et al. 1997; Waddington 1940). Though for many years this process was seen as unidirectional and irreversible, the idea that totipotent differentiation potential is completely lost during development was challenged by amphibian cloning studies in the 1960s (Gurdon 2006) and later put to rest by the cloning of Dolly (Campbell et al. 1996). Recent work by Yamanaka has demonstrated that differentiated cells can regain the potential to respond to cues and change cell type after treatment with defined transcription factors (Takahashi and Yamanaka 2006). Nonetheless, this process is still quite inefficient and questions remain regarding the global mechanisms at work during development to direct cell fate, as well as those acting in the reprogramming process to reverse it (Hochedlinger and Plath 2009).
Accompanying differentiation are specific changes in nuclear genome organization including chromatin structure, chromatin positioning in the nucleus, and the order in which different chromosomal regions are replicated during S-phase known as the replication timing program (Hiratani et al. 2008; Meshorer and Misteli 2006). Though research in recent years has brought to light many players involved in establishing and modifying chromatin organization (Li et al. 2007), no overarching mechanisms have been identified that can explain the connections between these molecular mechanisms and the seemingly concomitant higher-order reorganization events. Nevertheless, changes in higher-order organization correlate with changes in gene expression during development, suggesting that as yet undefined mechanisms regulating higher-order chromosomal structure may contribute to the differentiation process (Fraser and Bickmore 2007). Analysis of replication timing offers a unique approach to view the nuclear programming process during development because it provides a distinct functional property of chromosomes that is clearly regulated at the level of megabase-sized domains. Moreover, while regulatory mechanisms of replication timing remain elusive, evidence points to an intimate relationship between replication timing and chromatin packaging in the nucleus. Here, we review studies that underscore the importance of replication timing's connection to changes in the nucleus that occur during development.
Replication timing is connected to functional and spatial organization of chromatin
Recent genome-wide analyses of replication timing have confirmed that large blocks of chromatin replicate together (Desprat et al. 2009; Farkash-Amar et al. 2008; Hiratani et al. 2008; Karnani et al. 2007; MacAlpine et al. 2004; Schubeler et al. 2002; White et al. 2004; Woodfine et al. 2004), which we refer to as replication domains. These domains are replicated by synchronously firing origins in close enough proximity to each other to ensure that the length of each domain is replicated within a relatively short period of time (approximately 1 h in mammals; Gilbert and Gasser 2006). A comparison of these domains with Giemsa-stained chromomeric bands, which roughly demarcate regions of euchromatin and heterochromatin along the length of a chromosome, reveals general overlap of light-staining R bands with early-replicating domains and dark-staining G bands with late-replicating domains (Bickmore and Craig 1997; Craig and Bickmore 1993; Holmquist et al. 1982; Latt 1977). Accordingly, chromatin modifications characteristic of euchromatin such as histone acetylation overlap with early-replicating domains (Hiratani et al. 2009). Replication timing is also associated with three-dimensional genome organization in the nucleus. Spatial patterns of DNA replication in the nucleus change dramatically as cells move through S-phase, with early-replicating domains localized in the interior of the nucleus, whereas late-replicating domains are localized to the heterochromatin-rich nuclear and nucleolar peripheries as well as a few central heterochromatic blocks (Berezney et al. 2000). Importantly, this demonstrates a global coupling of sub-nuclear positioning and replication timing of different chromatin domains.
Expectedly, since chromatin structure is associated with transcriptional regulation, correlations between replication timing and gene expression have also been observed. Late-replicating domains packaged in heterochromatin and localized to the nuclear periphery tend to contain fewer genes than early-replicating domains and a much lower percentage of the genes within late-replicating domains are expressed relative to early-replicating regions (Hiratani et al. 2008). It is important to note that transcription correlates considerably better with replication timing over large chromosomal domains than at the level of individual genes (MacAlpine and Bell 2005; MacAlpine et al. 2004), suggesting greater transcriptional competence in early versus late-replicating domains. In fact, a recent study integrating the same reporter gene into many different chromosomal sites revealed a domain-wide regulatory mechanism influencing transcriptional output (Gierman et al. 2007). This study demonstrated that domains with high gene density and high GC content, which are features of early-replicating domains, showed higher reporter gene transcription. Naturally, however, genes will not be highly transcribed in the absence of gene-specific transcription factors regardless of domain structure, so a domain-wide regulatory mechanism would not be sufficient for transcription. Moreover, when considering regulation at the domain level, one should not be daunted by exceptional genes that appear to break general rules; additional mechanisms exist that locally fine-tune transcription, for example to overcome the condensed nature of a heterochromatic domain [(Hiratani et al. 2008) and references therein]. Collectively, these observations suggest that replication timing is fundamentally associated with establishment of the chromatin structure of broad regions, possibly affecting their accessibility to transcription machineries.
X chromosome inactivation as a paradigm for developmental regulation
The strongest evidence for developmental regulation of replication timing and its relationship to transcriptional regulation is the paradigm of X chromosome inactivation. In female mammalian somatic cells, the dosage-compensated, transcriptionally inactive X chromosome (Xi) is replicated late in S-phase, distinctly later than when the autosomes and its active X counterpart are replicated (Morishima et al. 1962). While some epigenetic events that accompany X-inactivation are species specific (Chow and Heard 2009), late replication is one of the most conserved features and has served as a cytological X-inactivation index for decades. During random X-inactivation in the embryo proper, the Xi undergoes a switch to late replication in the postimplantation epiblast, primarily during E5.8–6.3 (Takagi et al. 1982), meaning that the switch to late replication occurs prior to germ layer specification. Importantly, this narrow time window implies that a replication timing switch of the Xi may occur within a single cell cycle (Snow 1977). Accompanying late replication, the Xi becomes condensed and repositioned toward the nuclear periphery, forming a Barr body (Barr and Bertram 1949). This late-replicating condensed structure is subsequently maintained throughout development, underscoring the stability of this regulation (Chadwick and Willard 2003).
Embryonic stem cell (ESC) differentiation has provided a convenient in vitro model for studying X-inactivation. Conventional cytogenetic and molecular analyses have revealed that during female ESC differentiation, a switch to late replication occurs within 1–2 days after Xist RNA coating and the appearance of tri-methylation of histone H3 lysine 27 (H3K27me3) and di-methylation of H3K9 (H3K9me2) on the Xi (Chow and Heard 2009). A chromosome-wide histone H4 hypoacetylation and DNA methylation follows the replication timing switch [reviewed in Chow and Heard (2009)]. Analysis of individual genes suggested that transcriptional inactivation initiates immediately following Xist coating. However, recent chromosome-wide analyses reveal a more complex scenario. First, transcriptional silencing takes place over a span of 2–3 weeks and shows promoter-dependent regulation (Chow and Heard 2009; Lin et al. 2007). Secondly, cytogenetic studies of the Xi suggest the presence of two distinct types of repressed chromatin, one enriched for H3K27me3 and the other for H3K9me3 almost in a mutually exclusive manner (Chadwick and Willard 2004), suggesting that different domains undergo distinct epigenetic changes. It is not yet known how these domains relate to the replication timing changes along the length of the X-chromosome. Furthermore, the types and distribution of chromatin marks on the Xi are specific to certain species (Chow and Heard 2009). Together, there is a general relationship between changes that take place on the Xi, but some of these events may occur in a different order at different locations on the chromosome and the temporal order of these events with respect to the switch to late replication is difficult to define precisely. Nonetheless, controlled X-inactivation using an Xist transgene has defined a transition from a reversible initiation step to committed irreversible X-inactivation that coincides with the replication timing switch (Keohane et al. 1996; Wutz and Jaenisch 2000). Hence, it is difficult to formulate a precise hypothesis regarding causal relationships of replication timing to other chromatin changes, but late replication appears to be somehow related to the stability of the silenced state.
Developmental regulation on autosomes
Compared to the regulation of the X chromosome, relatively less is known about regulation of autosomes during development. Laborious work by many investigators identified a few dozen loci that replicate at different times in different cell lines (Gilbert 2002). From these studies, a general picture emerged that when a tissue-specific gene locus is subject to replication timing regulation, the locus is almost always early replicating in cell types in which it is expressed, whereas it is late replicating when transcriptionally silent. However, these studies also found many gene loci that remained early replicating in all cell types. Furthermore, most were inferred from the comparison of stable, transformed cell lines that represent different tissues, which may have acquired properties during long-term culture that are not seen in the tissues of origin. The key to solving these uncertainties was the advent of homogeneous differentiation systems that could elicit timing changes in response to media conditions (Hiratani et al. 2004; Perry et al. 2004). Moreover, genome-wide microarray technology and its application to replication studies (MacAlpine and Bell 2005) allowed for the possibility to generate a complete description of autosomal replication timing changes. In fact, recent genome-wide studies directly demonstrated that autosomal replication timing changes are widespread during mammalian development. Neural differentiation of mouse ESCs is accompanied by replication timing changes affecting ~20% of the genome, with smaller differentially replicating domains consolidating into larger coordinately replicated units (Hiratani et al. 2008). A similar fraction of the genome was found to differ in replication timing between embryonic versus wing disk cell lines in Drosophila (Schwaiger et al. 2009). Intriguingly, changes in replication timing were coordinated with transcription changes at the level of large chromosomal domains and rearrangements in sub-nuclear positioning (Hiratani et al. 2008; Williams et al. 2006). The relationship during differentiation demonstrates that changes in replication timing, transcription and sub-nuclear positioning are dynamically associated, and not related merely because they are correlates of static properties of the genome. Using a relatively synchronous differentiation system, the order of events could be addressed, revealing that the order of transcription vs. replication timing changes is locus-specific, whereas sub-nuclear position changes occurred only when replication timing changes traverse the mid-late stages of S-phase (Hiratani et al. 2010). Hence, there is a general coordination of events, but no specific kinetic relationship. Nonetheless, the fact that these associated properties change coordinately suggests a novel form of genome reorganization in the nucleus during differentiation.
These studies implied that genome-wide replication timing profiles are cell-type specific and consequently that 20% is almost certainly an underestimate of chromosomal regions subject to replication timing regulation during development. Consistently, recent genome-wide analyses of cell culture systems that model early mouse development indicate that replication timing regulation collectively affects at least 45% of the genome at some point during differentiation to different germ layers, creating cell-type-specific replication profiles (Hiratani et al. 2010). Interestingly, these experiments revealed that a significant number of early-to-late replication timing changes occurred in a lineage-independent manner and were completed at a stage equivalent to the postimplantation epiblast, which were subsequently stably maintained in downstream lineages (Hiratani et al. 2010). The distinction lies at the epiblast stage, with late postimplantation epiblast resembling downstream germ layer cell types, while early epiblast stage cells more closely resemble pluripotent cells of the inner cell mass (i.e. ESCs). Intriguingly, the replication timing switches in the epiblast occur during a period of little transcriptional change, implying that the replication switches are independent of large-scale changes in transcription. Moreover, lineage-independent, early-to-late replication timing changes coincide with movement of loci toward the nuclear periphery and were difficult to reverse once in place, being stably retained in cells returned to ESC medium and in cell lines that failed to fully revert to the induced pluripotent stem cell (iPSC) state in reprogramming experiments (i.e. cells known as partially reprogrammed iPSCs). In contrast, fully reprogrammed iPSCs reacquire a replication timing program indistinguishable from ESCs (Hiratani et al. 2008, 2010). These results suggest that lineage-independent autosomal replication timing switches occur at the same time and are as developmentally stable as late replication of the Xi. The similar time windows of events imply that the mechanisms regulating lineage-independent replication timing switches on the Xi and autosomes may cross-talk in the epiblast. However, additional lineage-specific replication timing changes continued to occur in more committed cell types, implying mechanisms exist that function throughout development.
Reconciling developmental regulation of replication timing with its relationship to static features of chromosomes
Studies of replication timing have identified a correlation to static features of chromosomes such as isochore GC content and chromosome banding patterns (Bickmore and Craig 1997; Craig and Bickmore 1993; Holmquist et al. 1982; Latt 1977). In fact, one report claimed a “perfect” match of replication timing to isochore GC content (Schmegner et al. 2007). Indeed, an attempt to reconcile static properties with the dynamic changes that occur during development is befuddling. However, in the case of isochore GC content, recent genome-wide analyses have resolved this dilemma by demonstrating that the relationship is far from perfect and is in fact quite cell-type specific (Hiratani et al. 2008, 2010). Moreover, the correlation is considerably stronger in mouse vs. human cells of the same type (T. Ryba, I.H. and DMG, unpublished).
In the case of R and G banding patterns, the question arises as to just how static they are across different tissues. Although the primary sequence of chromosomes has been shown to be a large determinant of banding patterns (Bobrow and Madan 1973; Korenberg and Engels 1978), other factors of chromosome structure must contribute as distinctive patterns can be seen between genetically identical active and inactive X chromosomes. However, to date, the only evidence that reverse/Giemsa (R/G) bands can change to coincide with developmental replication timing changes is during the inactivation of the X chromosome (Baranovskaya et al. 1972; Kanda 1973; Sarto et al. 1974). The most logical explanation for the apparent static coincidence of replication timing with R/G bands is that differences in replication timing encompass regions that are too small to detect by cytogenetic analyses. In fact, high-resolution banding methods have still not been applied to a systematic comparison of different tissues. Even those techniques with the highest resolution produce average band widths of 1.5 Mb (Yunis 1981), which still may not be sufficient to resolve replication timing switching domains (400–800 Kb). It is likely that a greater number of bands exist than have been resolved with existing cytogenetic methods, since bands are scored using a threshold staining intensity whereas a range of intensity exists within bands (Bickmore and Craig 1997). In fact, segments of bands with different intensities have been shown to replicate at different times (Ganner and Evans 1971). Hence, the question remains open as to whether or not cytogenetic methods of a resolution sufficient to detect replication domains would reveal tissue-specific chromosome banding patterns. Moreover, a simple inspection of the now-available genome-wide replication profiles (http://www.replicationdomain.org; Weddington et al. 2008) reveals a rather poor correspondence of replication timing to R and G bands. What will be necessary to resolve these issues will be a direct comparison of high-resolution banding patterns to replication timing profiles in the same cell line.
How do developmental cues impinge upon replication timing regulation?
The timing decision point (TDP) offers an opportunity for altering the replication program
One potential target of a developmentally driven toggle to modulate replication timing would be the timing decision point. After pre-replication complexes are loaded onto chromatin during telophase, the timing program for the subsequent S-phase is established at the TDP, typically 1–3 h into G1-phase in mammals (Dimitrova and Gilbert 1999) and at some point between mitosis and START in budding yeast (Raghuraman et al. 1997). The TDP was originally demonstrated in mammals by experiments introducing cell nuclei into Xenopus egg extracts. Nuclei isolated early in G1 could not maintain the normal program while those isolated later in G1 consistently maintained the regular temporal pattern of replication (Dimitrova and Gilbert 1999). The concept of the TDP implies that timing is re-established in each cell cycle and hence this cell cycle regulation may offer a point of intervention for developmentally regulated changes in replication timing.
Regulation is at the level of large chromosomal domains
While it may seem logical for replication timing changes to be exerted locally at the level of individual replication origins, either by activating or repressing a tissue-specific origin or by modifying the firing time of an active origin, this concept is difficult to reconcile with our current understanding of metazoan replication origins. First, replication origin selection is independent of replication timing regulation. Using the same Xenopus egg extract system described above, selection of origins was shown to take place at a G1 time point distinctively after the TDP (at the origin decision point; Dimitrova and Gilbert 1999; Dimitrova et al. 2002; Li et al. 2003; Wu and Gilbert 1996). In addition, developmental regulation of replication timing at the beta-globin locus is conserved between human and mouse, but origin site usage is very different; in human cells initiation is confined to one of two closely spaced origins (Aladjem et al. 1998; Kitsberg et al. 1993) while replication initiates from a dispersed set of origins in mice (Aladjem et al. 2002). Second, positions of initiation sites are quite flexible even among cells within a population of the same type (Aladjem 2007; Norio et al. 2005; Takebayashi et al. 2001). Hence, different cells use a different cohort of origins in each cell cycle, so that any mechanism would have to control timing regardless of individual cell origin choice. Taken together, a single-replicon level regulation of replication timing is difficult to reconcile with the contemporary view of metazoan replication origins. However, because our knowledge base on replication origins is still inadequate, regulation at the single-origin level cannot be totally excluded, particularly as an isolated mechanism in specific regions. In fact, a single very strong artificial initiation site was capable of eliciting a modest change in replication timing (Goren et al. 2008).
Cis regulation of domains and genomic context
Studies in budding yeast clearly show that cis-acting information exists that regulates the time of activation of origins independent of their initiation activity per se (Ferguson and Fangman 1992; Friedman et al. 1996). Consistently, a 200-bp cis element that enforces late replication of a neighboring origin has been identified in fission yeast (Yompakdee and Huberman 2004). However, the mechanism by which cis-acting sequences influence replication time is still unknown. In mammals, a deletion in the vicinity of the locus control region (LCR) of the human beta-globin gene locus has been shown to disrupt the domain's variable replication time in different cell types (Cimbora et al. 2000; Goren and Cedar 2003; Simon et al. 2001). It is thus reasonable to expect that cis-acting regulatory elements for replication timing exist in mammals. However, it has not been easy to pinpoint these sequences. For instance, sequences from within the beta-globin LCR itself that appear to be necessary and sufficient to confer replication timing regulation in the context of a transgene in mice are not necessary for timing in the native chromosomal context (Cimbora et al. 2000; Simon et al. 2001). Thus, cis regulation is dependent on the genomic context.
In agreement with the importance of genomic contexts, chromosomal domains that changed replication timing were AT-rich and above a certain threshold of long interspersed nuclear element 1 (LINE-1) transposon density (Hiratani et al. 2008), confirming an earlier study at the genome-wide scale (Hiratani et al. 2004). Our recent ongoing work provides a refined view (Hitarani et al. 2010): constitutively, early-replicating sequences (i.e. early in all cell types tested) were extremely GC rich, gene rich, and LINE-1 poor, whereas constitutively late-replicating sequences were extremely GC poor, gene poor, and LINE-1 rich. It appears that chromosomal domains with sequence properties intermediate between these two extremes are those subject to replication timing regulation during development. Potentially, subtle changes in the dosage of chromatin proteins that bind rather non-specifically to AT vs. GC-rich regions (as, for example, HMG-I/Y and H1 discussed above) could have a profound influence on the enrichment of those proteins across domains with intermediate GC content, particularly if these proteins are capable of spreading through positive feedback loops that seed multi-protein chromatin structures. Changes in protein dosage could be induced globally in the cell or locally by movement of a domain into a spatial compartment where such proteins are enriched or sequestered. In either case, a rapid domain-wide change in chromatin composition could be seeded by subtle changes in the binding of one or a few proteins. With genome-wide replication timing information in hand, chromosome engineering methods can now be employed to make targeted genetic deletions or insertions that address the interaction between cis-acting sequences, genomic environments, and sub-nuclear compartments.
Roles of replication timing regulation
The precise role of a replication timing program, and why this program is developmentally regulated remains to be elucidated. However, temporal regulation of genome duplication and the existence of multi-replicon domains are conserved from humans to budding and fission yeasts (Hayashi et al. 2007; McCune et al. 2008; Versini et al. 2003). DNA replication is centrally linked to many basic cellular processes that are regulated during the cell cycle and development, and defects in replication timing have been observed in various disease models [reviewed in (Hiratani and Gilbert 2009)]. Recent studies allow us to conclude that widespread developmental replication timing switches occur in flies (Schwaiger et al. 2009), mice (Hiratani et al. 2008), and humans (T. Ryba and D.M.G., unpublished). Moreover, there is significant conservation of the replication timing program when regions of conserved synteny from similar cell types are compared between human and mouse (T. Ryba and D.M.G., unpublished). This conservation is greater than the conservation of relative GC content between these same regions. Whatever its role, whether causal or reflective, replication timing is related to properties held in common across large chromosomal segments, and reflects developmental changes to these properties.
Since chromatin is assembled at the replication fork, the most appealing role for replication timing in regulating gene expression during development is to seed the assembly of different types of chromatin at different times during S-phase. In fact, reporter plasmids injected into early or late S-phase mammalian nuclei were assembled into hyper- or hypo-acetylated chromatin, respectively (Zhang et al. 2002). This result demonstrates that the environment within nuclei is significantly different at different times during S-phase. A recent follow-up study strengthens this scenario. Taking advantage of the fact that bovine papilloma virus plasmids replicate at different times in consecutive cell cycles (Gilbert and Cohen 1987), it was shown that hypoacetylated late-replicating chromatin becomes hyperacetylated when the mini-circle is replicated early in the subsequent cell cycle (Lande-Diner et al. 2009). Their results suggested that preacetylated histone H4 is actively deacetylated only during late S-phase, while unmodified histone H3 is actively acetylated by an unknown histone acetylase during early S. Interestingly, acetylation of H4K16, which is enriched in early-replicating DNA but depleted in late-replicating regions (Schwaiger et al. 2009), has been shown to prevent chromatin compaction (Corona et al. 2002; Robinson et al. 2008; Shogren-Knaak et al. 2006; Suganuma et al. 2008). Thus, while evidence is still limited, this longstanding hypothesis remains the most likely model that can place timing control as an upstream regulator of chromatin structure and explain the correlation observed in these two properties. In addition, such a system would be ideal for regulating developmental changes, allowing the local modifications to cis elements made at the TDP to spread broad high-order structural changes to domains on the order of several hundred kilobases in a single cell cycle.
Research in the laboratory of D.M.G. is supported by the NIH grant GM083337. We thank B. Chadwick for critical reading of the manuscript.
- Bickmore WA, Craig J (1997) Chromosome bands: patterns in the genome. Landes Bioscience, GeorgetownGoogle Scholar
- Desprat R, Thierry-Mieg D, Lailler N et al (2009) Predictable dynamic program of timing of DNA replication in human cells. Genome Res; in pressGoogle Scholar
- Gilbert DM, Gasser SM (2006) Nuclear structure and DNA replication. In: DePamphilis ML (ed) DNA replication and human disease. Cold Spring Harbor Laboratory, Cold Spring HarborGoogle Scholar
- Hiratani I, Ryba T, Itoh M et al (2010) Genome-wide dynamics of replication timing revealed by in vitro models of mouse embryogenesis. Genome Res; in pressGoogle Scholar
- Snow MHL (1977) Gastrulation in the mouse: growth and regionalization of the epiblast. J Embryol Exp Morphol 42:293–303Google Scholar
- Waddington CH (1940) Organisers and genes. Cambridge University, CambridgeGoogle Scholar