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Chromosome Research

, 17:1051 | Cite as

Complex genomic organization of Indian muntjac centromeric DNA

  • Ya-Ming Cheng
  • Tzai-Shiuan Li
  • Lie-Jiau Hsieh
  • Pei-Ching Hsu
  • Yueh-Chun Li
  • Chyi-Chyang Lin
Article

Abstract

A 69-kb Indian muntjac bacterial artificial chromosome (BAC) clone that screened positive for Cervid satellites I and IV was selected for complete sequence analysis and further characterization. The sequences of this BAC clone were found in the centromeres and in some interstitial sites of Indian muntjac chromosomes. Sequence analyses showed that the BAC clone contained a 14.5 kb Cervid satellite I-like DNA element and a 9 kb Cervid satellite IV-like DNA element. In addition, it contained 51 regions each organized in a complex fashion, with sequences homology to intersperse repetitive sequences such as LINEs, SINEs, LTRs, other published DNA elements, and unassigned sequences. The FISH patterns of seven non-satellite sequence elements generated from the BAC clone showed mainly specific to centromeres of the Indian muntjac representing novel centromeric DNAs of the species. Furthermore, FISH signals and Southern blot patterns of these elements suggest the existence of a not yet identified repetitive sequence with giant repeated monomers. Positive FISH signals of these elements were also detected in the centromeric regions of Formosan muntjac. This suggests that these newly identified non-Cervid satellite DNA sequences have been conserved in the centromere of the Formosan muntjac.

Keywords

centromere centromeric DNA satellite DNA Cervid satellite DNA BAC clone Indian muntjac 

Abbreviations

BAC

Bacterial artificial chromosome

FISH

Fluorescence in situ hybridization

LINEs

Long interspersed nucleotide elements

SINEs

Short interspersed nucleotide elements

LTRs

Long terminal repeats

Notes

Acknowledgements

This research was supported by grants from National Sciences Council, Taiwan (NSC96-2311-B-005-007-MY2 to Y-M Cheng; NSC95-2311-B-040-001 and NSC97-2311-B-006-003-MY3 to Y.-C Li; and NSC 97-2314-B-039-017 to C.C. Lin). A grant support from the Department of Medical Research, China medical University Hospital (DHR 94-050) to CCL is also acknowledged.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ya-Ming Cheng
    • 1
  • Tzai-Shiuan Li
    • 2
  • Lie-Jiau Hsieh
    • 2
  • Pei-Ching Hsu
    • 3
  • Yueh-Chun Li
    • 2
  • Chyi-Chyang Lin
    • 3
    • 4
  1. 1.Department of AgronomyNational Chung Hsing UniversityTaichungTaiwan
  2. 2.Department of Biomedical Sciences, China Medical University and Department of Medical ResearchChung Shan Medical University HospitalTaichungTaiwan
  3. 3.Department of Medical Research, China Medical University Hospital and Graduate Institute of Basic Medical SciencesChina Medical UniversityTaichungTaiwan
  4. 4.Laboratory for Chromosome ResearchChina Medical University and HospitalTaichungTaiwan

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