Advertisement

Chromosome Research

, Volume 13, Issue 2, pp 145–156 | Cite as

The evolutionary origin of insect telomeric repeats, (TTAGG) N

  • Magda Vítková
  • Jiří Král
  • Walther Traut
  • Jan Zrzavý
  • František Marec
Article

Abstract

RNA polymerase II is responsible for transcription of most eukaryotic genes, but, despite exhaustive analysis, little is known about how it transcribes natural templates in vivo. We studied polymerase dynamics in living Chinese hamster ovary cells using an established line that expresses the largest (catalytic) subunit of the polymerase (RPB1) tagged with the green fluorescent protein (GFP). Genetic complementation has shown this tagged polymerase to be fully functional. Fluorescence loss in photobleaching (FLIP) reveals the existence of at least three kinetic populations of tagged polymerase: a large rapidly-exchanging population, a small fraction resistant to 5,6-dichloro-1-β-D-ribofuranosylbenzimidazole (DRB) but sensitive to a different inhibitor of transcription (i.e. heat shock), and a third fraction sensitive to both inhibitors. Quantitative immunoblotting shows the largest fraction to be the inactive hypophosphorylated form of the polymerase (i.e. IIA). Results are consistent with the second (DRB-insensitive but heat-shock-sensitive) fraction being bound but not engaged, while the third (sensitive to both DRB and heat shock) is the elongating hyperphosphorylated form (i.e. IIO).

Key words

Arthropoda chromosomes Onychophora phylogeny Tardigrada telomere 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Magda Vítková
    • 1
    • 2
  • Jiří Král
    • 3
  • Walther Traut
    • 4
  • Jan Zrzavý
    • 1
    • 2
  • František Marec
    • 1
    • 2
  1. 1.Faculty of Biological SciencesUniversity of South BohemiaČeskéCzech Republic
  2. 2.Institute of Entomology ASCR, BranišovskáČeské BudějoviceCzech Republic
  3. 3.Faculty of SciencesCharles UniversityCzech Republic
  4. 4.Institut für BiologieUniversität zu LübeckLübeckGermany

Personalised recommendations