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Chromosome Research

, Volume 13, Issue 3, pp 249–257 | Cite as

Investigating chromosome organization with genomic microarrays

  • Kathryn Woodfine
  • Nigel P. Carter
  • Ian Dunham
  • Heike Fiegler
Article

Abstract

DNA microarrays are increasingly being used to investigate the functional role of chromatin. These studies are enhanced by the development of high-resolution arrays covering either the whole genome or specific regions of selected chromosomes with large insert clones, PCR products or oligonucleotides of around 100 bp or less. In combination with chromatin immunoprecipitation, this approach allows identification of protein binding for transcription factors, proteins involved in DNA replication and repair as well as sites of chromatin modification. Furthermore, by application of S phase fractions to genomic microarrays, replication timing can be estimated. Thus, microarrays can provide new information about chromosome structure and gene regulation.

Key words

DNA microarray DNA replication immunoprecipitation transcription factor 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Kathryn Woodfine
    • 1
  • Nigel P. Carter
    • 2
  • Ian Dunham
    • 2
  • Heike Fiegler
    • 2
  1. 1.Department of Medical and Molecular GeneticsGKT School of MedicineLondonUK
  2. 2.The Wellcome Trust Sanger InstituteWellcome Trust Genome CampusCambridgeUK

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