Spermidine Exhibits Protective Effects Against Traumatic Brain Injury


Traumatic brain injury (TBI) causes permanent neurological and cognitive impairments. Effective pharmacological interventions remain elusive. Spermidine is a polyamine compound found in our body that may play a role in brain development and congenital function. In this study, we aimed to investigate the therapeutic potential of spermidine for TBI. We employed experimental closed head injury (CHI) model to evaluate the protective function of spermidine on brain injury. We assessed the neurobehavioral function recovery using Neurologic Severity Score (NSS) and Morris water maze test. At histological level, we evaluated the improvement on brain edema, brain–blood barrier integrity, and cell apoptosis. We also measured inflammatory cytokines and brain injury biomarkers to monitor the treatment outcomes. Last, we correlated the level of spermidine with CHI animal model and TBI patients with different levels of severity. Spermidine administration post-CHI was found effectively to accelerate NSS improvement and shorten latency in maze test. We observed consistent improvements in brain edema, BBB function, and cell death in spermidine-treated group. Inflammatory cytokines and TBI biomarkers, e.g., S100B, MBP and CFAP were reduced significantly in treatment group. Interestingly, inhibiting spermidine synthesis influenced the neurobehavioral recovery in CHI mice. ODC1, a rate-limiting enzyme for spermidine synthesis, was found lower in CHI mice. Serum level of spermidine was significantly lower in TBI patients with severe pathological scores. Spermidine pathway may carry an endogenous role in pathophysiological process of CHI. For the first time, we demonstrated that administrating spermidine may provide a new treatment for TBI.

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Jianxing Huang, Heping Zhang, Jinning Zhang, Huiping Yu, Zhizhong Lin, and Yonghui Cai did the experiments and analyzed the data; Heping Zhang and Jinning Zhang designed the study and wrote the manuscript:. All authors approved the final submission.

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Correspondence to Heping Zhang or Jinning Zhang.

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The animal study and the clinical study were approved by the Ethics Committee in Quanzhou First Hospital Affiliated to Fujian Medical University.

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The animal study followed the guide for the Care and Use of Laboratory Animals (Eighth Edition, NIH). The clinical study complied with the Declaration of Helsinki, Ethical Principles for Medical Research Involving Human Subjects.

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Huang, J., Zhang, H., Zhang, J. et al. Spermidine Exhibits Protective Effects Against Traumatic Brain Injury. Cell Mol Neurobiol 40, 927–937 (2020). https://doi.org/10.1007/s10571-019-00783-4

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  • Spermidine
  • Traumatic head injury
  • Closed head injury
  • ODC1