Abstract
Overcoming neurite inhibition is integral for restoring neuronal connectivity after CNS injury. Actin dynamics are critical for neurite growth cone formation and extension. The tropomyosin family of proteins is a regarded as master regulator of actin dynamics. This study investigates tropomyosin isoform 3.1 (Tpm3.1) as a potential candidate for overcoming an inhibitory substrate, as it is known to influence neurite branching and outgrowth. We designed a microfluidic device that enables neurons to be grown adjacent to an inhibitory substrate, Nogo-66. Results show that neurons, overexpressing hTpm3.1, have an increased propensity to overcome Nogo-66 inhibition. We propose Tpm3.1 as a potential target for promoting neurite growth in an inhibitory environment in the central nervous system.
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Acknowledgements
This work was supported by Project Grant APP1083209 from the Australian National Health and Medical Research Council (NHMRC) (T.F.) and Discovery Project Grant DP180101473 from the Australian Research Council (ARC) (T.F.). We thank Tamara Tomanić (UNSW Sydney) for her constructive feedback and critical reading of the manuscript. This work was performed (in part) at the NSW and South Australian node of the Australian National Fabrication Facility under the National Collaborative Research Infrastructure Strategy to provide nano- and microfabrication facilities for Australia’s researchers. M.E.W. would like to acknowledge the support of the Australian Research Council through Discovery Project Grants (DP170103704 and DP180103003) and the National Health and Medical Research Council through the Career Development Fellowship (APP1143377).
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TF, MEW and TB supervised the project. TF, HS, AH-B and SF designed the research. HS, AH-B, SF and MB performed the research and analysed the data. TF, HS, AH-B and SF wrote the paper. TF, HS, AH-B, AKS, MB and NT edited the paper. All authors read and approved the final manuscript.
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The authors declare that the research was conducted in the absence of any commercial, financial or non-financial relationships that could be construed as a potential conflict of interest.
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All procedures were conducted in accordance with the Australian Code of Practice for the Care and Use of Animals for Scientific Purposes and were approved by the University of New South Wales Animal Care and Ethics Committee.
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Stefen, H., Hassanzadeh-Barforoushi, A., Brettle, M. et al. A Novel Microfluidic Device-Based Neurite Outgrowth Inhibition Assay Reveals the Neurite Outgrowth-Promoting Activity of Tropomyosin Tpm3.1 in Hippocampal Neurons. Cell Mol Neurobiol 38, 1557–1563 (2018). https://doi.org/10.1007/s10571-018-0620-7
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DOI: https://doi.org/10.1007/s10571-018-0620-7