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IGFBP6 Regulates Cell Apoptosis and Migration in Glioma

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Abstract

The insulin-like growth factor binding protein 6 (IGFBP6), as an inhibitor of IGF-II actions, plays an important role in inhibiting survival and migration of tumor cells. In our study, we intended to demonstrate the biological function of IGFBP6 in the development of glioma and its clinical significance. Firstly, Western blot and immunohistochemistry revealed that the expression of IGFBP6 inversely correlated with glioma grade. Secondly, multivariate analysis with the Cox proportional hazards model and Kaplan–Meier analysis indicated that IGFBP6 could be an independent prognostic factor for the survival of glioma patients. In addition, overexpression of IGFBP6 induced glioma cell apoptosis, and depletion of IGFBP6 had the opposite action. Finally, overexpression of IGFBP6 inhibited migration of glioma cells, and depletion of IGFBP6 had the opposite action. Together our findings suggest that IGFBP6 might be an important regulator and prognostic factor for glioma.

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Acknowledgments

This work was supported by the National Natural Science Foundation of Jiangsu province (No. BK20130386), the Chinese Projects for Postdoctoral Science Funds (No. 2015M571792), and the Jiangsu Planned Projects for Postdoctoral Research Funds (No. 1402200C).

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    Authors and Affiliations

    1. Department of Neurosurgery, The Affiliated Hospital of Nantong University, Xisi Road No. 20, Nantong, 226001, People’s Republic of China

      Qingfeng Huang, Jianhong Shen, Jinlong Shi & JianGuo Chen

    2. Department of Radiation Oncology and Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, 226001, Jiangsu, People’s Republic of China

      Yuanqi Bei, Chaoyan Shen, Xiaojie Xia & Bin Ji

    3. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, People’s Republic of China

      Yuanqi Bei, Chaoyan Shen, Peng Xu & Xiaojie Xia

    4. Department of Intensive Care Unit, Affiliated Cancer Hospital of Nantong University, Nantong, 226361, People’s Republic of China

      Yuanqi Bei

    5. Department of Neurosurgery, Affiliated Wuxi Second Hospital of Nanjing Medical University, Wuxi, 214002, People’s Republic of China

      Hao Chang

    6. Department of Neurosurgery, The First Affiliated Hospital of Suzhou University, Suzhou, 215000, People’s Republic of China

      Li Xu

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    1. Yuanqi Bei
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    2. Qingfeng Huang
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    Correspondence to JianGuo Chen.

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    Yuanqi Bei, Qingfeng Huang, Bin Ji and Jianguo Chen have contributed equally to this work.

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    Bei, Y., Huang, Q., Shen, J. et al. IGFBP6 Regulates Cell Apoptosis and Migration in Glioma. Cell Mol Neurobiol 37, 889–898 (2017). https://doi.org/10.1007/s10571-016-0426-4

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    • DOI: https://doi.org/10.1007/s10571-016-0426-4

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