Cellular and Molecular Neurobiology

, Volume 34, Issue 4, pp 491–499 | Cite as

Spatiotemporal Pattern of RNA-Binding Motif Protein 3 Expression After Spinal Cord Injury in Rats

  • Wei Zhao
  • Dawei Xu
  • Gang Cai
  • Xinhui Zhu
  • Ming Qian
  • Wei Liu
  • Zhiming Cui
Original Research


RNA-binding motif protein 3 (RBM3) belongs to a very small group of cold inducible proteins with anti-apoptotic and proliferative functions. To elucidate the expression and possible function of RBM3 in central nervous system (CNS) lesion and repair, we performed a spinal cord injury (SCI) model in adult rats. Western blot analysis revealed that RBM3 level significantly increased at 1 day after damage, and then declined during the following days. Immunohistochemistry further confirmed that RBM3 immunoactivity was expressed at low levels in gray and white matters in normal condition and increased at 1 day after SCI. Besides, double immunofluorescence staining showed RBM3 was primarily expressed in the neurons and a few of astrocytes in the normal group. While after injury, the expression of RBM3 increased both in neurons and astrocytes at 1 day. We also examined the expression profiles of proliferating cell nuclear antigen (PCNA) and active caspase-3 in injured spinal cords by western blot. Importantly, double immunofluorescence staining revealed that cell proliferation evaluated by PCNA appeared in many RBM3-expressing cells and rare caspase-3 was observed in RBM3-expressing cells at 1 day after injury. Our data suggested that RBM3 might play important roles in CNS pathophysiology after SCI.


Spinal cord injury RNA-binding motif protein 3 Proliferating cell nuclear antigen Apoptosis Rat 



This work was supported by the National Natural Scientific Foundation of China Grant (No. 81300955), Projects of Nantong (No. BK2011013 and No. BK2012075).

Conflict of interest

The authors declare no conflict of interest associated with this work.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Wei Zhao
    • 1
    • 2
  • Dawei Xu
    • 1
  • Gang Cai
    • 2
  • Xinhui Zhu
    • 1
  • Ming Qian
    • 2
  • Wei Liu
    • 1
  • Zhiming Cui
    • 1
  1. 1.Department of OrthopaedicsThe Second Affiliated Hospital of Nantong UniversityNantongPeople’s Republic of China
  2. 2.Department of NeurosurgeryThe Second Affiliated Hospital of Nantong UniversityNantongPeople’s Republic of China

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