Cellular and Molecular Neurobiology

, Volume 32, Issue 8, pp 1231–1242 | Cite as

Comparison of Trophic Factors Changes in the Hippocampal CA1 Region Between the Young and Adult Gerbil Induced by Transient Cerebral Ischemia

  • Bing Chun Yan
  • Joon Ha Park
  • Sung Koo Kim
  • Jung Hoon Choi
  • Choong Hyun Lee
  • Ki-Yeon Yoo
  • Young-Geun Kwon
  • Young-Myeong Kim
  • Jong-Dai Kim
  • Moo-Ho Won
Original Research


In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89 %) were damaged 4 days after ischemia–reperfusion (I–R) in the adult; however, in the young, about 59 % of pyramidal neurons were damaged 7 days after I–R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I–R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I–R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I–R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.


Young animal Ischemia–reperfusion injury Pyramidal neurons More delayed neuronal death 



The authors would like to thank Mr. Seung Uk Lee and Ms. Hyun Sook Kim for their technical help in this study. This study was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NO2011-0022812), and by a grant (2010K000823) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Bing Chun Yan
    • 1
  • Joon Ha Park
    • 1
  • Sung Koo Kim
    • 2
  • Jung Hoon Choi
    • 3
  • Choong Hyun Lee
    • 4
  • Ki-Yeon Yoo
    • 5
  • Young-Geun Kwon
    • 6
  • Young-Myeong Kim
    • 7
  • Jong-Dai Kim
    • 8
  • Moo-Ho Won
    • 1
  1. 1.Department of Neurobiology, School of MedicineKangwon National UniversityChuncheonSouth Korea
  2. 2.Department of Pediatrics, Kangnam Sacred Heart Hospital, College of MedicineHallym UniversitySeoulSouth Korea
  3. 3.Department of Anatomy, College of Veterinary MedicineKangwon National UniversityChuncheonSouth Korea
  4. 4.Department of Anatomy and Physiology, College of PharmacyDankook UniversityCheonanSouth Korea
  5. 5.Department of Oral Anatomy, College of Dentistry and Research Institute of Oral BiologyGangneung-Wonju National UniversityGangneungSouth Korea
  6. 6.Department of Biochemistry, College of Life Science and BiotechnologyYonsei UniversitySeoulSouth Korea
  7. 7.Vascular System Research Center and Department of Molecular and Cellular Biochemistry, School of MedicineKangwon National UniversityChuncheonSouth Korea
  8. 8.Division of Food Biotechnology, School of BiotechnologyKangwon National UniversityChuncheonSouth Korea

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