Abstract
Hepatocyte growth factor (HGF) and its receptor, c-Met, are widely expressed in the developing brain. HGF also known as scatter factor enhances cell proliferation and cell growth, and stimulates cell migration and motility. Neurons and glia produced in the neuroepithelium migrate along radial glial fibers into the cortical plate. Reelin, a glycoprotein which is produced by Cajal–Retzius cells in the marginal zone directs neuronal migration indirectly via the radial glial cells. It has been demonstrated that Disabled 1 functions downstream of reelin in a tyrosin kinase signal transduction pathway that controls appropriate cell positioning in the developing brain. In this study, administration of HGF on reelin and Disabled 1 expression in the cerebral cortex has been studied. Using Western blot, it was shown that the expression of reelin and Disabled 1 is increased in response to infusion of HGF when compared to control group. It is concluded that HGF is essential for reelin and Disabled 1 expression in the cerebral cortex of the newborn mouse. Moreover, this method may be applied to the other factors, allowing identification of molecules involved in neural cell migration.
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Achim CL, Katyal S, Wiley CA, Shiratori M, Wang G, Oshika E, Petersen BE, Li JM, Michalopoulos GK (1997) Expression of HGF and cMet in the developing and adult brain. Brain Res Dev Brain Res 102:299–303
Bar I, Goffinet AM (2000) Evolution of cortical lamination: the reelin/Dab1 pathway. Novartis Found Symp 228:114–125
Barnabé-Heider F, Miller FD (2003) Endogenously produced neurotrophins regulate survival and differentiation of cortical progenitors via distinct signaling pathways. J Neurosci 23:5149–5160
Birchmeier C, Gherardi E (1998) Developmental roles of HGF/SF and its receptor, the c-Met tyrosine kinase. Trends Cell Biol 8:404–410
Caton A, Hacker A, Naeem A, Livet J, Maina F, Bladt F, Klein R, Birchmeier C, Guthrie S (2000) The branchial arches and HGF are growth-promoting and chemoattractant for cranial motor axons. Development 127:1751–1766
D’Arcangelo G (2001) The role of the Reelin pathway in cortical development. Symp Soc Exp Biol 53:59–73
Darmanto W, Inouye M, Hayasaka S, Takagishi Y, Ogawa M, Mikoshiba K, Murata Y (1998) Disturbed Purkinje cell migration due to reduced expression of Reelin by X-irradiation in developing rat cerebellum. Biol Sci Space 12:254–255
Duan X, Kang E, Liu CY, Ming GL, Song H (2008) Development of neural stem cell in the adult brain. Curr Opin Neurobiol 18:108–115
Gilmore EC, Herrup K (2000) Cortical development: receiving reelin. Curr Biol 10:R162–R166
Graziani A, Gramaglia D, Cantley LC, Comoglio PM (1991) The tyrosine-phosphorylated hepatocyte growth factor/scatter factor receptor associates with phosphatidylinositol 3-kinase. J Biol Chem 266:22087–22090
Honda S, Kagoshima M, Wanaka A, Tohyama M, Matsumoto K, Nakamura T (1995) Localization and functional coupling of HGF and c-Met/HGF receptor in rat brain: implication as neurotrophic factor. Brain Res Mol Brain Res 32:197–210
Jung W, Castren E, Odenthal M, Vande Woude GF, Ishii T, Dienes HP, Lindholm D, Schirmacher P (1994) Expression and functional interaction of hepatocyte growth factor-scatter factor and its receptor c-met in mammalian brain. J Cell Biol 126(2):485–494
Lan F, Xu J, Zhang X, Wong VW, Li X, Lu A, Lu W, Shen L, Li L (2008) Hepatocyte growth factor promotes proliferation and migration in immortalized progenitor cells. Neuroreport 19:765–769
Miyan JA, Nabiyouni M, Zendah M (2003) Development of the brain, a vital role for cerebrospinal fluid. Can J Physiol Pharmacol 81:317–328
Nayeri F, Nilsson I, Hagberg L, Brudin L, Roberg M, Söderström C, Forsberg P (2000) Hepatocyte growth factor levels in cerebrospinal fluid: a comparison between acute bacterial and nonbacterial meningitis. J Infect Dis 181:2092–2094
Nicholson C (1999) Signals that go with the flow. Trends Neurosci 22:143–145
Powell EM, Mars WM, Levitt P (2001) Hepatocyte growth factor/scatter factor is a motogen for interneurons migrating from the ventral to dorsal telencephalon. Neuron 30:79–89
Rice DS, Curran T (2001) Role of the reelin signaling pathway in central nervous system development. Annu Rev Neurosci 24:1005–1039
Royal I, Fournier TM, Park M (1997) Differential requirement of Grb2 and PI3-kinase in HGF/SF-induced cell motility and tubulogenesis. J Cell Physiol 173:196–201
Sarnat HB, Flores-Sarnat L (2002) Cajal-Retzius and subplate neurons, their role in cortical development. Eur J Paediatr Neurol 6:91–97
Sobeih MM, Corfas G (2002) Extracellular factors that regulate neuronal migration in the central nervous system. Int J Dev Neurosci 20:349–357
Supèr H, Soriano E, Uylings HB (1998) The functions of the preplate in development and evolution of the neocortex and hippocampus. Brain Res Brain Res Rev 27:40–64
Trommsdorff M, Gotthardt M, Hiesberger T, Shelton J, Stockinger W, Nimpf J, Hammer RE, Richardson JA, Herz J (1999) Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2. Cell 97:689–701
Uehara Y, Minowa O, Mori C, Shiota K, Kuno J, Noda T, Kitamura N (1995) Placental defect and embryonic lethality in mice lacking hepatocyte growth factor/scatter factor. Nature 373:702–705
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This study was supported in part by the University of Guilan.
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Mashayekhi, F., Gholizadeh, L. Administration of Hepatocyte Growth Factor Increases Reelin and Disabled 1 Expression in the Mouse Cerebral Cortex: An In Vivo Study. Cell Mol Neurobiol 31, 1267–1270 (2011). https://doi.org/10.1007/s10571-011-9728-8
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DOI: https://doi.org/10.1007/s10571-011-9728-8