Abstract
In order to explore whether the apoptosis in ischemia–reperfusion injury could be affected by Ginkgo biloba extract (GBE) and the free radical scavenger GBE could suppress this affection. Rabbits were randomly divided into sham group, ischemia group, ischemia–reperfusion group (1, 6, 24, 48 h), the drug group (1, 6, 24, 48 h). Measure the rate of apoptosis by flow cytometry, the caspase 9 and apoptosis-inducing factor (AIF) in the cytoplasm and serum by ELISA. Compared with the sham group and ischemia group, the reperfusion group increased the rate of apoptosis, the caspase 9 and AIF in serum have a peak at 24 h after reperfusion, in the cytoplasm the peak at 6 h.GBE inhibit performance has the systemic and local aspects. The apoptosis of nerve cells after spinal cord ischemia–reperfusion has the relationship with the mitochondrial caspase-dependent and caspase-independent pathways and both the local and systemic role. GBE inhibits nerve cell apoptosis by these ways.
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Weizhuo Wang, Lei Lin, Fengtao Li, and Xin Wang contributed equally to this work.
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Cheng, B., Wang, W., Lin, L. et al. The Change of the Spinal Cord Ischemia–Reperfusion Injury in Mitochondrial Passway and the Effect of the Ginkgo biloba Extract’s Preconditioning Intervention. Cell Mol Neurobiol 31, 415–420 (2011). https://doi.org/10.1007/s10571-010-9634-5
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DOI: https://doi.org/10.1007/s10571-010-9634-5