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Cellular and Molecular Neurobiology

, Volume 30, Issue 8, pp 1315–1319 | Cite as

Association of SNAREs and Calcium Channels with the Borders of Cytoskeletal Cages Organizes the Secretory Machinery in Chromaffin Cells

  • Cristina J. Torregrosa-Hetland
  • José Villanueva
  • Inmaculada López-Font
  • Virginia Garcia-Martinez
  • Amparo Gil
  • Virginia Gonzalez-Vélez
  • Javier Segura
  • Salvador Viniegra
  • Luis M. Gutiérrez
Review Paper

Abstract

In chromaffin cells, SNARE proteins, forming the basic exocytotic machinery are present in membrane clusters of 500–600 nm in diameter. These microdomains containing both SNAP-25 and syntaxin-1 are dynamic and the expression of altered forms of SNAREs modifies not only their motion but also the mobility of the associated granules. It is also clear that SNARE microdomain location defines the place for individual vesicle fusion and that the alteration of cluster dynamics affects the fusion process itself. Interestingly, these SNARE patches colocalize with the borders of F-actin cages forming the cytoskeletal cortical network, and these borders also contain clusters of L- and P/Q type calcium channels. The organization of the secretory machinery in association with the borders of cytoskeletal cages seems to be an effective way to promote fast coupling between calcium entry and catecholamine release as demonstrated with the use of mathematical secretory models.

Keywords

Chromaffin cells SNAREs Calcium channels Cytoskeleton Exocytosis Active sites 

Notes

Acknowledgments

This work was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN, Fondos FEDER: BFU2008-02154/BFI), and the Generalitat Valenciana (GRUPOS03/040 and ACOMP09/036). CJT-H was recipient of and FPU fellowship.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Cristina J. Torregrosa-Hetland
    • 1
  • José Villanueva
    • 1
  • Inmaculada López-Font
    • 1
  • Virginia Garcia-Martinez
    • 1
  • Amparo Gil
    • 2
  • Virginia Gonzalez-Vélez
    • 3
  • Javier Segura
    • 4
  • Salvador Viniegra
    • 1
  • Luis M. Gutiérrez
    • 1
  1. 1.Instituto de NeurocienciasCentro Mixto CSIC-Universidad Miguel HernándezAlicanteSpain
  2. 2.Departamento de Matemática Aplicada y Ciencias de la ComputaciónUniversidad de CantabriaSantanderSpain
  3. 3.Área de Química Aplicada, Universidad Autónoma Metropolitana-AzcapotzalcoMexicoMexico
  4. 4.Departamento de Matemáticas, Estadística y ComputaciónUniversidad de CantabriaSantanderSpain

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