Cellular and Molecular Neurobiology

, Volume 30, Issue 7, pp 985–990 | Cite as

3-Mercaptopropionic Acid-Induced Seizures Decrease NR2B Expression in Purkinje Cells: Cyclopentyladenosine Effect

  • E. Girardi
  • J. Auzmendi
  • N. Charó
  • M. B. Gori
  • M. Castro
Short Communication



NR2B Cerebellum Epilepsy Adenosine Purkinje cells 3-Mercaptopropionic acid Seizures 



This study was supported by grants of UBACYT from the Universidad de Buenos Aires, and CONICET (PIP no 5798) from the Consejo Nacional de Investigaciones Científicas y Técnicas.


  1. Auzmendi J, González N, Girardi E (2009) The NMDAR subunit NR2B is modified in hippocampus after repetitive seizure. Neurochem Res 34:819–826CrossRefPubMedGoogle Scholar
  2. Blumenfeld H, Varghese GI, Purcaro MJ, Motelow JE, Enev M, McNally KA, Levin AR, Hirsch LJ, Tikofsky R, Zubal IG, Paige AL, Spencer SS (2009) Cortical and subcortical networks in human secondarily generalized tonic-clinic seizures. Brain 132:999–1012CrossRefPubMedGoogle Scholar
  3. Cooke PM, Snider RS (1955) Some cerebellar influences on electrically induced cerebral seizures. Epilepsia 4:19–28CrossRefPubMedGoogle Scholar
  4. Cull-Candy S, Brickey S, Farrant M (2001) NMDA receptors subunits: diversity, development and disease. Curr Opin Neurobiol 11:327–335CrossRefPubMedGoogle Scholar
  5. Dingledine R, Borges K, Bowie D, Traynellis F (1999) The glutamate receptor ion channels. Pharmacol Rev 51:7–62PubMedGoogle Scholar
  6. Dunwiddie TV, Masino SA (2001) The role and regulation of adenosine in the central nervous system. Annu Rev Neurosci 24:31–55CrossRefPubMedGoogle Scholar
  7. Erickson MA, Haburćák M, Smukler L, Dunlap K (2007) Altered functional expression of Purkinje cell calcium channels precedes motor dysfunction in tottering mice. Neuroscience 150:547–555CrossRefPubMedGoogle Scholar
  8. Gan YC, Connoll MB, Steinbok P (2007) Epilepsy associated with a cerebellar arachnoid cyst: seizure control following fenestration of the cyst. Childs Nerv Syst 24:14–125CrossRefGoogle Scholar
  9. Gielen M, Le Goff A, Stroebel D, Johnson JW, Neyton J, Paoletti P (2008) Structural rearrengements of NR1/NR2A NMDA receptors during allosteric inhibition. Neuron 57:80–93CrossRefPubMedGoogle Scholar
  10. Giraldez L, Girardi E (1998) Modification of 3H-MK801 binding to rat brain NMDA receptors after the administration of a convulsant drug and an adenosine analogue. A quantitative autoradiographic study. Neurochem Res 23:1327–1336CrossRefPubMedGoogle Scholar
  11. Giraldez L, Zanetti F, Antonelli MC, Rodríguez de Lores Arnaiz G (1998) CNS Adenosine A1 receptors are altered after the administration of convulsant 3-mercaptopropionic acid and cyclopentyladenosine: an autoradiographic study. Neurochem Res 23:175–181CrossRefPubMedGoogle Scholar
  12. Girardi E, Perez Raffo G, Rodríguez de Lores Arnaiz G (1989) A study of 5′-nucleotidase activity in subcellular fractions of rat cerebellum fractions after the administration of the convulsant 3-mercaptopropionic acid. Molec Chem Neuropath 11:65–75CrossRefGoogle Scholar
  13. Girardi E, Ramos AJ, Vanore G, Brusco A (2004) Astrocytic response in hippocampus and cerebral cortex in an experimental epilepsy model in rat. Neurochem Res 29:371–377CrossRefPubMedGoogle Scholar
  14. Girardi E, Canitrot J, Antonelli M, González N, Coirini H (2007) Differential expression of cerebellar metabotropic glutamate receptors mGluR2/3 and mGluR4a after the administration of a convulsant drug and the adenosine analogue cyclopentyladenosine. Neurochem Res 32:1120–1128CrossRefPubMedGoogle Scholar
  15. Goldowitz D, Hamre K (1998) The cells and molecules that make a cerebellum. Trends Neurosci 21:375–382CrossRefPubMedGoogle Scholar
  16. Harvey AS, Jayakar P, Duchowny M, Resnick T, Altman N, Renfroe JB (1996) Hemifacial seizures and cerebellar ganglioma: an epilepsy syndrome of infancy with seizures of cerebellar origin. Ann Neurol 40:91–98CrossRefPubMedGoogle Scholar
  17. Ito M (2006) Cerebellar circuitry as a neuronal machine. Prog Neurobiol 78:272–303CrossRefPubMedGoogle Scholar
  18. Jarvis MF, Williams M (1990) Adenosine in central nervous system function. In: William M (ed) Adenosine and adenosine receptors. Humana Press, Clifton New Jersey, pp 423–474Google Scholar
  19. Kakegawa W, Tsuzuki K, Iino M, Ozawa S (2003) Functional NMDA receptors channels generated by NMDAR2B gene transfer in rat cerebellar Purkinje cells. Eur J Neurosci 17:887–891CrossRefPubMedGoogle Scholar
  20. Köhr G (2006) NMDA receptor function; subunit composition versus spatial contribution. Cell Tissue Res 326:439–446CrossRefPubMedGoogle Scholar
  21. Llansola M, Sanchez-Perez A, Cauli O, Felipo V (2005) Modulation of NMDA receptors in the cerebellum.1. Properties of the NMDa receptor that modulate its function. Cerebellum 4:154–161CrossRefPubMedGoogle Scholar
  22. Lowry DH, Rosebrough N, Farr A et al (1951) Protein measurement with the Folin phenol reagent. J. Biol Chem 193:265–275PubMedGoogle Scholar
  23. Mesiwala AH, Kuratani JD, Avellino AM, Roberts TS, Sotero MA, Ellenboge NRG (2002) Focal motor seizures with secondary generalization arising in the cerebellum. Case report and review of the literature. J Neurosurg 97:190–196CrossRefPubMedGoogle Scholar
  24. Paoletti P, Neyton J (2007) NMDA receptor subunits: function and pharmacology. Curr Opin Pharmacol 7:39–47CrossRefPubMedGoogle Scholar
  25. Piochon C, Irinopoulous T, Brusciano D, Bailly Y, Mariani J, Levenes C (2007) NMDA receptor contribution to the climbing fiber response in the adult mouse Purkinje cell. J Neurosci. 27:10797–10809CrossRefPubMedGoogle Scholar
  26. Renzi M, Farrant M, Cull-Candy SG (2007) Climbing-fibre activation of NMDA receptors in Purkinje cells of adult mice. J Physiol 585:91–101CrossRefPubMedGoogle Scholar
  27. Reynolds YJ, Palmer AM (1991) Regional variations in [3H]MK801 binding to rat brain N-methyl-D-aspartate receptors. J Neurochem 56:1731–1740CrossRefPubMedGoogle Scholar
  28. Ribeiro JA, Sebastiao AM, de Mendonca A (2003) Participation of adenosine receptors in neuroprotection. Drug News Perspect 16:80–86CrossRefPubMedGoogle Scholar
  29. Rodríguez de Lores Arnaiz G, Alberici de Canal M, De Robertis E (1972) Alteration of GABA system and Purkinje cells in rat cerebellum by the convulsant 3-mercaptopropionic acid. J Neurochem 19:1379–1385CrossRefGoogle Scholar
  30. Thompson CL, Drewery DL, Atkins HD, Stephenson FA, Chazot PL (2000) Immunohistochemical localization of N-methyl-D-aspartate receptor NR1, NR2A, NR2B and NR2C/D subunits in the adult mammalian cerebellum. Neurosci Lett 283:85–88CrossRefPubMedGoogle Scholar
  31. Velasco F, Carrillo-Ruiz JD, Brito F, Velasco M, Velasco AL, Marquez I, Davis R (2005) Double-blind, randomized controlled pilot study of bilateral cerebellar stimulation for treatment of intractable motor seizures. Epilepsia 46:1071–1081CrossRefPubMedGoogle Scholar
  32. Wall MJ, Atterbury A, Dale N (2007) Control of basal extracellular adenosine concentration in rat cerebellum. J Physiol 582:137–151CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • E. Girardi
    • 1
  • J. Auzmendi
    • 1
  • N. Charó
    • 1
  • M. B. Gori
    • 1
  • M. Castro
    • 1
  1. 1.Instituto de Biología Celular y Neurociencia, Facultad de MedicinaUniversidad de Buenos Aires and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

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