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Colchicine-Induced Apoptosis Was Prevented by Glycogen Synthase Kinase-3 Inhibitors in PC12 Cells

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Abstract

The purpose of this study was to examine whether glycogen synthase kinase-3 (GSK-3) is involved in colchicine-induced cell death in PC12 cells by using GSK inhibitors. Colchicine increased apoptotic cell death with morphological changes characterized by cell shrinkage and nuclear condensation or fragmentation. GSK-3 inhibitors such as alsterpaullone, SB216763, and AR-A014418 prevented colchicine-induced cell death and caspase-3 activation. These results suggest that colchicine induces caspase-dependent apoptotic cell death and that GSK-3 activation is involved in cell death in PC12 cells.

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Acknowledgments

This study was supported in part by a special in-house research grant from Hokuriku University.

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Authors and Affiliations

  1. Department of Clinical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan

    Tsuneo Takadera, Yu Nakajima & Yuki Kanai

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  1. Tsuneo Takadera
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  2. Yu Nakajima
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  3. Yuki Kanai
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Correspondence to Tsuneo Takadera.

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Takadera, T., Nakajima, Y. & Kanai, Y. Colchicine-Induced Apoptosis Was Prevented by Glycogen Synthase Kinase-3 Inhibitors in PC12 Cells. Cell Mol Neurobiol 30, 863–868 (2010). https://doi.org/10.1007/s10571-010-9514-z

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  • DOI: https://doi.org/10.1007/s10571-010-9514-z

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