Cyclin D1 Immunoreactivity in Meningiomas
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Objective Cyclin D1 is an important nuclear protein required for progression of cells through the G1 phase of the cell cycle. The proliferative potential of meningiomas has been studied using various proliferative markers. However, there have been only few published studies evaluating Cyclin D1 immunoreactivity in meningiomas. Purpose of the study The aim of our study was to analyze the Cyclin D1 expression in meningiomas and correlate it both with proliferation markers Ki67 and PCNA, and with meningiomas of WHO grade. Material and methods We evaluated immunoreactivity for proliferative markers (Cyclin D1, Ki-67, and PCNA) in a consecutive series of 64 meningioma samples obtained from patients who underwent surgical resection because of cerebral or spinal meningiomas. Immunohistochemical staining with Ki-67, PCNA, and Cyclin D1 was performed using the microwave processing procedure and LSAB+ methodology. The number of positive cells for each antibody has been determined and shown in percentage in relation to 1000 counted cells. Results All meningioma samples showed immunostaining for Ki-67, PCNA, and Cyclin D1 antibodies. The Cyclin D1 scores exhibited a close correlation with Ki-67 and PCNA immunostaining (P < 0.01). Some meningiomas (15 cases) showed a combination of nuclear and cytoplasmatic (fine granular) Cyclin D1 immunoreactivity. All proliferative indexes have been in positive correlation with meningioma grade. Conclusion Our comparative study of proliferative markers in meningiomas demonstrated Cyclin D1 as a very useful proliferative marker in meningiomas.
KeywordsMeningiomas Proliferative indices Cyclin D1 Ki67 PCNA Immunohistochemistry
The authors wish to thank Prof. Dr. Dubravka Cvetkovic-Dozic and Prof. Milica Skender-Gazibara and Prof. Slobodan Dozic for useful suggestions and helpful discussion of the manuscript. We also thank Milan Gajic for the crucial help with statistical analysis of data.
- Arber N, Gammon MD, Hibshoosh H, Britton JA, Zhang Y, Schonberg JB, Roterdam H, Fabian I, Holt PR, Weinstein B (1999) Overexpression of cyclin D1 occurs in both squamous carcinomas and adenocarcinomas of the esophagus and in adenocarcinomas of the stomach. Hum Pathol 30:1087–1092PubMedCrossRefGoogle Scholar
- Kato JY, Matsuoka M, Strom DK, Sherr CJ (1994) Regulation of cyclin D-dependent kinase 4 (cdk4) by cdk4-activating kinase. Mol Cell Biol 14(4):2713–2721Google Scholar
- Korshuno A, Shishkina L, Golanov A (2002) DNA Topoisomerase II-a and Cyclin A. Immunoexpression in meningiomas and its prognostic significance an analysis of 263 cases. Arch Pathol Lab Med 126:1079–1086Google Scholar
- Louis DN, Scheithauer BW, Budka H, von Deimling A, Kepes JJ (2000) Meningiomas: pathology and genetics of tumours of the nervous system. In: Kleihues P, Cavenee WK (eds) World Health Organization classification of tumours. IARC Press, Lyon, pp 176–184Google Scholar
- Milenkovic S, Berisavac I, Bojović V, Orlić M, Stanković D, Simić A (2005) Multiple spinal meningiomas with positive PR-case report. Mater Med 21(2):105–109Google Scholar