Morroniside Prevents Peroxide-induced Apoptosis by Induction of Endogenous Glutathione in Human Neuroblastoma Cells
(1) Morroniside belongs to an extensive group of natural iridorid glycosides. In the present study, using human neuroblastoma SH-SY5Y cells, we have investigated the protective effects of this compound on modifications in endogenous reduced glutathione (GSH), intracellular oxygen species (ROS) and apoptotic death on H2O2-mediated cytoxicity. (2) Incubation of cells with morroniside led to a significant dose-dependent elevation of cellular GSH accompanied by a marked protection against H2O2-mediated toxicity. Morroniside at 1–100 μM inhibited the formation of ROS and the activation of caspase-3 and 9, and the upregulation of Bcl-2, whereas no significant change occurred in Bax levels. (3) The results indicated that the anti-oxidative and anti-apoptotic properties render this natural compound potentially protective against H2O2-induced cytotoxicity. (4) This study suggested that intracellular GSH appeared to be an important factor in morroniside-mediated cytoprotection against H2O2-toxicity in SH-SY5Y cells.
KeywordsMorroniside Antioxidant Apoptosis Human neuroblastoma cell Iridorid glycoside Neurodegenerative disorders Cornus officinalis
This research was supported by the grants from the Program Foundation: Chinese “973” program foundation (2003CB517104); Beijing Natural Science Foundation, China (No: 7062031, 7050001, 7032013); Beijing Science and Technology Committee Program Foundation, China (D0204003000031, D0206001043191); Beijing Superiority Talented Man Foundation, China (No: 2004100501816); Base-Clinical Foundation of Capital Medical University, China (No: 2004JL02, 2006JL09); Key Laboratory for Neurodegenerative Disease Ministry of Education Foundation, China (No:2007).
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