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Morroniside Prevents Peroxide-induced Apoptosis by Induction of Endogenous Glutathione in Human Neuroblastoma Cells

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Abstract

(1) Morroniside belongs to an extensive group of natural iridorid glycosides. In the present study, using human neuroblastoma SH-SY5Y cells, we have investigated the protective effects of this compound on modifications in endogenous reduced glutathione (GSH), intracellular oxygen species (ROS) and apoptotic death on H2O2-mediated cytoxicity. (2) Incubation of cells with morroniside led to a significant dose-dependent elevation of cellular GSH accompanied by a marked protection against H2O2-mediated toxicity. Morroniside at 1–100 μM inhibited the formation of ROS and the activation of caspase-3 and 9, and the upregulation of Bcl-2, whereas no significant change occurred in Bax levels. (3) The results indicated that the anti-oxidative and anti-apoptotic properties render this natural compound potentially protective against H2O2-induced cytotoxicity. (4) This study suggested that intracellular GSH appeared to be an important factor in morroniside-mediated cytoprotection against H2O2-toxicity in SH-SY5Y cells.

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Acknowledgement

This research was supported by the grants from the Program Foundation: Chinese “973” program foundation (2003CB517104); Beijing Natural Science Foundation, China (No: 7062031, 7050001, 7032013); Beijing Science and Technology Committee Program Foundation, China (D0204003000031, D0206001043191); Beijing Superiority Talented Man Foundation, China (No: 2004100501816); Base-Clinical Foundation of Capital Medical University, China (No: 2004JL02, 2006JL09); Key Laboratory for Neurodegenerative Disease Ministry of Education Foundation, China (No:2007).

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Authors and Affiliations

  1. Department of Pharmacology, Xuan-Wu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing, 100053, China

    Wen Wang, Lin Li, Houxi Ai & Ci Liu

  2. School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, China

    Wenting Huang, Fangling Sun & Yi An

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  1. Wen Wang
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  2. Wenting Huang
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  3. Lin Li
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  5. Fangling Sun
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Correspondence to Lin Li.

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Wang, W., Huang, W., Li, L. et al. Morroniside Prevents Peroxide-induced Apoptosis by Induction of Endogenous Glutathione in Human Neuroblastoma Cells. Cell Mol Neurobiol 28, 293–305 (2008). https://doi.org/10.1007/s10571-007-9168-7

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  • DOI: https://doi.org/10.1007/s10571-007-9168-7

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