Cellular and Molecular Neurobiology

, Volume 26, Issue 7–8, pp 1341–1351 | Cite as

Impact of Ginkgo Biloba Extract EGb 761 on Ischemia/Reperfusion – Induced Oxidative Stress Products Formation in Rat Forebrain

  • A. Uríková
  • E. Babušíková
  • D. Dobrota
  • A. Drgová
  • P. Kaplán
  • Z. Tatarková
  • J. Lehotský


Dysbalance in reactive oxygen/nitrogen species is involved in the pathogenesis of cerebral ischemia/reperfusion injury (IRI). Ginkgo biloba extract (Egb 761) pre-treatment was used to observe potential antioxidant/neuroprotective effect after global ischemia/reperfusion. Egb 761 significantly decreased the level of lipoperoxidation (LPO) in rat forebrain total membrane fraction (homogenate) induced by in vitro oxidative stress (Fe2++H2O2). In animals subjected to four-vessel global ischemia for 15 min and 2–24 h reperfusion the EGb pretreatment slightly decreased LPO in forebrain homogenate. However, as detected in EGb treated group, the LPO-induced lysine conjugates are attenuated in comparison to non-treated IRI animals. EGb significantly improved parameters which indicate forebrain protein oxidative damage after IRI. The intensity of tryptophane fluorescence was increased by the 18.2% comparing to non-treated IRI group and bityrosine fluorescence was significantly decreased in ischemic (21%) and 24 h reperfused (15.9%) group in comparison non-treated IRI group. In addition, the level of total free SH- groups in pre-treated animals was significantly higher comparing to non-treated animals. Our results indicate that extract of EGb 761 has potent antioxidant activity and could play a role to attenuate the IRI-induced oxidative protein modification and lipoperoxidation in the neuroprotective process.


extract of ginkgo biloba cerebral ischemia rat-protein oxidation lipid peroxidation fluorescence 



Presented as Abstract on the 5th International Symposium on Experimental and Clinical Neurobiology, Tatranska Lomnica-Stara Lesna, Slovak Republic, September 2005. This study was supported by a research grants: VEGA No. 034/03 and 3380/06 and APVT No. 51-013802.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • A. Uríková
    • 1
  • E. Babušíková
    • 1
  • D. Dobrota
    • 1
  • A. Drgová
    • 1
  • P. Kaplán
    • 1
  • Z. Tatarková
    • 1
  • J. Lehotský
    • 1
  1. 1.Department of Medical BiochemistryJessenius Faculty of Medicine, Comenius UniversityMartinSlovakia

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