Summary
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1.
Literature data indicate that serotonin induces the long-term potentiation of glutamate (Glu) response in molluscan neurons. The aim of present work was to elucidate whether cyclic nucleotides can cause the same effect.
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2.
Experiments were carried out on isolated neurons of the edible snail (Helix pomatia) using a two-microelectrode voltage-clamp method.
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3.
In the majority of the cells examined, the application of Glu elicited a Cl−-current. The reversal potential (Er) of this current lied between −35 and −55 mV in different cells.
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4.
Picrotoxin, a blocker of Cl−-channels, suppressed this current equally on both sides of Er. Furosemide, an antagonist of both Cl−-channels and the Na+/K+/Cl−-cotransporter, had a dual effect on Glu-response: decrease in conductance, and shift of Er to negative potentials.
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5.
A short-term (2 min) cell treatment with 8-Br-cAMP or 8-Br-cGMP caused long-term (up to 30 min) change in Glu-response. At a holding potential of −60 mV, which was close to the resting level, an increase in Glu-activated inward current was observed. This potentiation seems to be related to the right shift of Er of Glu-activated Cl−-current rather than to the increase in conductance of Cl−-channels. The blocking effect of picrotoxin rested after 8-Br-cAMP treatment.
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6.
The change in the Cl−-homeostasis as a possible mechanism for the observed effect of cyclic nucleotides is discussed.
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Bukanova, J.V., Solntseva, E.I. & Skrebitsky, V.G. Cyclic Nucleotides Induce Long-Term Augmentation of Glutamate-Activated Chloride Current in Molluscan Neurons. Cell Mol Neurobiol 25, 1185–1194 (2005). https://doi.org/10.1007/s10571-005-8371-7
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DOI: https://doi.org/10.1007/s10571-005-8371-7