, Volume 25, Issue 4, pp 2589–2598 | Cite as

Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells

  • Hyeon Joo KimEmail author
  • Rodrigo Castañeda
  • Tong Ho Kang
  • Satoshi Kimura
  • Masahisa Wada
  • Ung-Jin KimEmail author
Original Paper


Herein, we present a new process for the formation of cell spheroids in three-dimensional culture systems using cellulose hydrogel film. Transparent cellulose hydrogel film were prepared via dissolution-regeneration with a LiOH/urea aqueous solution and methanol. The cell viability of the cellulose hydrogel film was equivalent to that of cell tissue culture plate, revealing no cytotoxicity. Spheroids of human adipose-derived stem cells (hASCs) were successfully formed on cellulose hydrogel film. Spheroid size strongly depended on the cell seeding density and culture time, becoming larger with increases in both factors. Cell differentiation simultaneously progressed favorably with the spheroid formation of hASCs. These results suggested that cellulose hydrogel film could provide a new option with regard to tissue engineering applications.


Cellulose Hydrogel film Adipose-derived stem cells Spheroid Osteogenic 



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2015R1D1A1A01058918 and NRF-2017R1D1A1B03030800).

Supplementary material

10570_2018_1732_MOESM1_ESM.docx (937 kb)
Supplementary material 1 (DOCX 937 kb)


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of BiotechnologyKyung Hee UniversityGiheung-gu Yongin-siRepublic of Korea
  2. 2.Department of Plant and Environmental New Resources, College of Life SciencesKyung Hee UniversityGiheung-gu, Yongin-siRepublic of Korea
  3. 3.Department of Biomaterials Science, Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  4. 4.Division of Forest and Biomaterials Science, Graduate School of AgricultureKyoto UniversitySakyo-ku, KyotoJapan

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