, Volume 23, Issue 5, pp 3187–3198 | Cite as

In vitro biodegradability of bacterial cellulose by cellulase in simulated body fluid and compatibility in vivo

  • Baoxiu Wang
  • Xiangguo Lv
  • Shiyan Chen
  • Zhe Li
  • Xiaoxiao Sun
  • Chao Feng
  • Huaping Wang
  • Yuemin Xu
Original Paper


Bacterial cellulose (BC) has great potential for use as a tissue scaffold due to its unique structure and properties including high tensile strength and good biocompatibility. However, poor biodegradability of BC in the human body may be a key disadvantage limiting its application in the field. In this paper, we developed a simple absorption method to prepare biodegradable cellulase/BC materials. The morphology, structure, degradation ratio and mechanical properties during the degradation process were characterized and investigated. In vitro studies reveal that the BC material degraded gradually in simulated body fluid within 24 weeks and the degradation rate could be adjusted by modulating the cellulase content. The mechanical properties indicate the cellulase/BC material could maintain tensile strength for as long as 24 days during the degradation process. Muscle-derived cells were seeded on the cellulase/BC material to evaluate the cytotoxicity, using LIVE/DEAD® viability/cytotoxicity assay and H&E staining. In vivo biocompatibility was evaluated by subcutaneous implantation using a dog model for 1, 2, 3 and 4 weeks. These results demonstrate that the cellulase/BC material had good in vitro and in vivo biocompatibility.


Bacterial cellulose Cellulase Biodegradation Tissue scaffold 



This work was financially supported by the National Natural Science Foundation of China (51273043, 51573024 and 81370795), the Fundamental Research Funds for the Central Universities and DHU Distinguished Young Professor Program.

Compliance with ethical standards

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Supplementary material

10570_2016_993_MOESM1_ESM.docx (468 kb)
Supplementary material 1 (DOCX 467 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Key Laboratory of Textile Science and Technology (Ministry of Education), College of Materials Science and EngineeringDonghua UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Urology, Affiliated Sixth People’s HospitalShanghai Jiaotong UniversityShanghaiPeople’s Republic of China

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