, Volume 21, Issue 3, pp 1699–1708 | Cite as

Thermoresponsive hybrid hydrogel of oxidized nanocellulose using a polypeptide crosslinker

Original Paper


Thermoresponsive hybrid nanocellulose hydrogels were prepared from a mixture of oxidized nanocellulose and elastin-like polypeptide (ELP). Positively charged ELP was used as a polymeric crosslinker for conjugation with negatively charged nanocellulose. Hydrogel formation was triggered by a simple increase in temperature, and the hydrogel was reversibly returned to the liquid phase by decreasing temperature. Surface potential measurement confirmed the electrostatic properties of oxidized nanocellulose and ELP molecules. The surface morphology of hydrogels was observed by atomic force microscopy and field emission-scanning electron microscopy. Conformational changes in the ELP/nanocellulose hybrid were characterized by circular dichroism. The ELP/nanocellulose hybrid hydrogel was noncytotoxic and suitable for encapsulating cells, indicating its potential for biomedical applications.


Bacterial cellulose Polypeptide Thermo-responsiveness Hydrogel 



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning (Grant Number 2013023612). We also acknowledge support from the Research Institute for Agriculture and Life Sciences.

Supplementary material

10570_2014_208_MOESM1_ESM.doc (33 kb)
Supplementary material 1 (DOC 33 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of Biosystems and Biomaterials Science and EngineeringSeoul National UniversitySeoulKorea

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