Effects of lignin on the ionic-liquid assisted catalytic hydrolysis of cellulose: chemical inhibition by lignin
The production of cellulose-derived biofuels and biochemicals, such as bioalcohols and bioplastics, from lignocellulose requires the isolation of cellulose by lignin removal or delignification processes. While the remaining lignin and its phenolic fragments have been reported to inhibit the biological conversion of cellulose, we observed that the catalytic hydrolysis of cellulose also can be inhibited most likely because of an associative interaction between cellulose and lignin. The associative interaction between cellulose and the functional groups of lignin was proven by gel-permeation-chromatography measurement of regenerated mixtures of lignin and cellulose which simulate the lignocellulose-derived cellulose containing lignin as an impurity. Chemical bonds between cellulose and lignin were hypothesized using lignin model compounds containing known functionalities such as hydroxyl, methoxy, phenyl, allyl, and carboxyl groups in order to explain the effects of lignin on the hydrolysis of cellulose. The yield of glucose from cellulose dropped when carboxylic and hydroxyl groups were present possibly because of the formation of ether and ester bonds between the lignin and cellulose. These observations may help develop the chemical processes and therefore convert the inedible biomass resource of lignocellulose-based cellulose containing lignin and its derivatives to the valuable fuels and chemicals.
KeywordsLignin Hydrolysis Catalysis Ether Ester Hydroxyl Carboxyl
This research was supported by ‘Creative Allied Project (CAP)’ grant funded by the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST). This work was also supported by the National Research Foundation of Korea grant funded by the Korean Government (MSIP) (2013, University-Institute cooperation program).
- Dwiatmoko AA, Choi JW, Suh DJ, Suh Y-W, Kung HH (2010) Understanding the role of halogen-containing ionic liquids in the hydrolysis of cellobiose catalyzed by acid resins. Appl Catal A 387(1–2):209–214Google Scholar
- Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass. Laboratory analytical procedureGoogle Scholar
- Whetten RW, MacKay JJ, Sederoff RR (1998) Recent advances in understanding lignin biosynthesis. Annu Rev Plant Phys 49(1):585–609Google Scholar