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Antimicrobial porous hybrids consisting of bacterial nanocellulose and silver nanoparticles

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Abstract

The increasing resistance of pathogens and bacteria is a serious problem in the medical treatment of wounds and injuries. Therefore, new therapeutic agents are not solely based on antibiotics, but also on the use of antimicrobial metal nanoparticles. In this paper we present an innovative method to prepare porous hybrids consisting of bacterial nanocellulose (BNC) and silver nanoparticles (AgNPs). The stepwise modification is based on fairly simple chemical reactions already described for two-dimensional cellulose films. We transferred this method to the three-dimensional, porous network of BNC leading to an antimicrobial activation of its surface. Compared to former approaches, the ultrafine network structure of BNC is less damaged by using mild chemicals. The amount and distribution of the AgNPs on the modified BNC was investigated using scanning electron microscopy. The AgNPs are firmly immobilized on the top and bottom surface of the BNC by chemical interactions. Their size and quantity increase with an increasing concentration of AgNO3 and extended reaction time in the AgNO3 solution. A strong antimicrobial activity of the BNC-AgNP hybrids against Escherichia coli was detected. Furthermore, agar diffusion tests confirmed that this activity is restricted to the modified dressing itself, avoiding a release of NPs into the wound. Therefore, the produced hybrids could be potentially suited as novel antimicrobial wound dressings.

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Acknowledgments

The authors thank R. Zimmermann, INNOVENT e.V. Technologieentwicklung Jena, for his kind support during the antimicrobial studies and D. Reichmann for agar diffusion tests (Department of Dermatology and Allergology). F.W. acknowledges the DBU (Deutsche Bundesstiftung Umwelt) for financial support. C.W., D.K. and F.A.M. are grateful for the funding of this work by the Thuringian Ministry of Education, Science and Culture (B714-10032) and the European Fund for Regional Development.

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Authors and Affiliations

  1. Institute of Materials Science and Technology (IMT), Friedrich-Schiller-University of Jena, Loebdergraben 32, 07743, Jena, Germany

    Sabrina Berndt, Falko Wesarg & Frank A. Müller

  2. Department of Pharmaceutical Technology, Institute of Pharmacy, Friedrich-Schiller-University of Jena, Otto-Schott-Strasse 41, 07745, Jena, Germany

    Dana Kralisch

  3. Department of Dermatology and Allergology, University Medical Center Jena, Erfurter Strasse 35, 07743, Jena, Germany

    Cornelia Wiegand

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  1. Sabrina Berndt
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  2. Falko Wesarg
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  3. Cornelia Wiegand
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  4. Dana Kralisch
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  5. Frank A. Müller
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Corresponding author

Correspondence to Dana Kralisch.

Additional information

S. Berndt and F. Wesarg contributed equally to this work. D. Kralisch and F. A. Müller are members of the Jena Center for Soft Matter.

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Berndt, S., Wesarg, F., Wiegand, C. et al. Antimicrobial porous hybrids consisting of bacterial nanocellulose and silver nanoparticles. Cellulose 20, 771–783 (2013). https://doi.org/10.1007/s10570-013-9870-1

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