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Cellulose

, Volume 20, Issue 2, pp 771–783 | Cite as

Antimicrobial porous hybrids consisting of bacterial nanocellulose and silver nanoparticles

  • Sabrina Berndt
  • Falko Wesarg
  • Cornelia Wiegand
  • Dana Kralisch
  • Frank A. Müller
Original Paper

Abstract

The increasing resistance of pathogens and bacteria is a serious problem in the medical treatment of wounds and injuries. Therefore, new therapeutic agents are not solely based on antibiotics, but also on the use of antimicrobial metal nanoparticles. In this paper we present an innovative method to prepare porous hybrids consisting of bacterial nanocellulose (BNC) and silver nanoparticles (AgNPs). The stepwise modification is based on fairly simple chemical reactions already described for two-dimensional cellulose films. We transferred this method to the three-dimensional, porous network of BNC leading to an antimicrobial activation of its surface. Compared to former approaches, the ultrafine network structure of BNC is less damaged by using mild chemicals. The amount and distribution of the AgNPs on the modified BNC was investigated using scanning electron microscopy. The AgNPs are firmly immobilized on the top and bottom surface of the BNC by chemical interactions. Their size and quantity increase with an increasing concentration of AgNO3 and extended reaction time in the AgNO3 solution. A strong antimicrobial activity of the BNC-AgNP hybrids against Escherichia coli was detected. Furthermore, agar diffusion tests confirmed that this activity is restricted to the modified dressing itself, avoiding a release of NPs into the wound. Therefore, the produced hybrids could be potentially suited as novel antimicrobial wound dressings.

Keywords

Antimicrobial activity Silver nanoparticles Escherichia coli Bacterial nanocellulose 

Notes

Acknowledgments

The authors thank R. Zimmermann, INNOVENT e.V. Technologieentwicklung Jena, for his kind support during the antimicrobial studies and D. Reichmann for agar diffusion tests (Department of Dermatology and Allergology). F.W. acknowledges the DBU (Deutsche Bundesstiftung Umwelt) for financial support. C.W., D.K. and F.A.M. are grateful for the funding of this work by the Thuringian Ministry of Education, Science and Culture (B714-10032) and the European Fund for Regional Development.

Supplementary material

10570_2013_9870_MOESM1_ESM.doc (2.6 mb)
Supplementary material 1 (DOC 2703 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Sabrina Berndt
    • 1
  • Falko Wesarg
    • 1
  • Cornelia Wiegand
    • 3
  • Dana Kralisch
    • 2
  • Frank A. Müller
    • 1
  1. 1.Institute of Materials Science and Technology (IMT)Friedrich-Schiller-University of JenaJenaGermany
  2. 2.Department of Pharmaceutical Technology, Institute of PharmacyFriedrich-Schiller-University of JenaJenaGermany
  3. 3.Department of Dermatology and AllergologyUniversity Medical Center JenaJenaGermany

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