, Volume 21, Issue 1, pp 407–416 | Cite as

Utilization of a biphasic oil/aqueous cellulose nanofiber membrane bioreactor with immobilized lipase for continuous hydrolysis of olive oil

Original Paper


A simple method is proposed to fabricate a biphasic lipase-immobilized cellulose membrane bioreactor with high enzyme loading and activity retention. This bioreactor was assembled with electrospun cellulose nanofiber membranes that were fixed in a spiral form and wound to increase their specific surface area. To improve the catalytic efficiency of the immobilized enzymes, the supports went through alkaline hydrolysis, NaIO4 oxidation and pentaethylenehexamine modification before covalently binding the lipase. Enzyme loading could reach 28.9 mg/g with the highest activity retention of 44.3 % for the immobilized lipases. The effects of the operational variables, namely the organic phase flow rate, aqueous phase flow rate and substrate concentration, on the performance of this bioreactor were investigated with continuous hydrolysis of olive oil. It was found that under optimum operational conditions, 100 % hydrolysis conversion of olive oil was achieved after 9 organic phase circulations at 10.5 mL/min organic phase flow rate, 600 mL/min aqueous phase flow rate and using a substrate of pure olive oil. Nanofiber membrane bioreactors offer potential as applications for various lipase-catalyzing reactions in industrial productions.


Cellulose Nanofiber membrane Enzyme immobilization Lipase Biphasic enzyme-immobilized membrane bioreactor 



The authors are grateful to the financial support from the Fundamental Research Funds for the Central Universities (Grant no. 2013QNA4090), the National Natural Science Foundation of China (Grant no. 21274126), and the National “Twelfth Five-Year” Plan for Science & Technology Support of China (Grant no. 2012BAI08B01).

Supplementary material

10570_2013_148_MOESM1_ESM.doc (109 kb)
Supplementary material 1 (DOC 109 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and EngineeringZhejiang UniversityHangzhouChina

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