, Volume 19, Issue 3, pp 761–768 | Cite as

Immobilization of gelatin onto natural nanofibers for tissue engineering scaffold applications without utilization of any crosslinking agent

  • Chuan Gao
  • Tao Yan
  • Kerong Dai
  • Yizao Wan
Original Paper


Bacterial cellulose was oxidized by periodate oxidation to give rise to 2,3-dialdehyde bacterial cellulose (DABC) with 60.3 ± 0.5 % aldehyde content, which was further reacted with gelatin (Gel) for the immobilization of Gel to form DABC/Gel nanocomposites. The scanning electron microscopy and transmission electron microscopy revealed that DABC/Gel exhibited the refined 3D nano-network structures and the average thickness of Gel coatings in the composites was about 75 nm. FTIR and XPS were utilized to analyze the functional groups and chemical states of DABC/Gel nanocomposites. The results inferred that Gel was fixed on DABC nanofibers via the Schiff base reaction between –NH2 in Gel and –CHO in DABC backbone. NIH3T3 mice fibroblast cells were used for determining the cytocompatibility of the scaffolds. The morphology of the cells was observed through optical inverted microscopy. The results show that DABC/Gel can be used as scaffold material in tissue engineering.


Gelatin Nanofibers Composites Tissue engineering 



This work is supported by the National Natural Science Foundation of China (Grants 50673076 and 50539060). Financial support was also from the State Key Basic Research (973) Program (Grant 2007CB936100) and the National Hi-Tech Research Development (863) Program (2009AA03Z311).


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.School of Materials Science and EngineeringTianjin UniversityTianjinPeople’s Republic of China
  2. 2.Department of Pharmacy ServicTianjin Huanhu HospitalTianjinPeople’s Republic of China
  3. 3.Institute of Health Sciences, Shanghai Institutes for Biological SciencesChinese Academy of Sciences and Shanghai JiaoTong University School of MedicineShanghaiPeople’s Republic of China
  4. 4.TianjinPeople’s Republic of China

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