Utilization and transport of l-arabinose by non-Saccharomyces yeasts
- 224 Downloads
l-Arabinose is one of the sugars found in hemicellulose, a major component of plant cell walls. The ability to convert l-arabinose to ethanol would improve the economics of biomass to ethanol fermentations. One of the limitations for l-arabinose fermentation in the current engineered Saccharomyces cerevisiae strains is poor transport of the sugar. To better understand l-arabinose transport and use in yeasts and to identify a source for efficient l-arabinose transporters, 165 non-Saccharomyces yeast strains were studied. These yeast strains were arranged into six groups based on the minimum time required to utilize 20 g/L of l-arabinose. Initial transport rates of l-arabinose were determined for several species and a more comprehensive transport study was done in four selected species. Detailed transport kinetics in Arxula adeninivorans suggested both low and high affinity components while Debaryomyces hansenii var. fabryii, Kluyveromyces marxianus and Pichia guilliermondii possessed a single component, high affinity active transport systems.
KeywordsNon-conventional yeast l-Arabinose utilization Sugar transport Mutagenesis
This work was funded by the United States Department of Energy’s Office of the Biomass Program, the Corn Refiners Association, and the National Corn Growers Association. We thank C. Kurtzman for providing some of the strains used in this study.
- Barnett JA, Payne RW, Yarrow D (2000) Yeasts: characteristics and identification. Cambridge University Press, CambridgeGoogle Scholar
- Cirillo VP (1968) Galactose Transport in Saccharomyces cerevisiae I. Nonmetabolized sugars as substrates and inducers of the galactose transport system. J Bacteriol 95:1727–1731Google Scholar
- Kou SC, Christensen MS, Cirillo VP (1970) Galactose transport in Saccharomyces cerevisiae II. Characteristics of galactose uptake and exchange in galactokinaseless cells. J Bacteriol 103:671–678Google Scholar
- Kurtzman CP, Robnett CJ (1997) Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35:1216–1223Google Scholar
- Sturgeon RJ (1984) Arabinose. In: Bergmeyer HU (ed) Methods of enzymatic analysis, 3rd edn. Verlag Chemie, Weinheim, pp 427–431Google Scholar