Cell Biology and Toxicology

, 27:413 | Cite as

Induction of apoptosis in murine leukemia by diarylheptanoids from Curcuma comosa Roxb.

  • Surawat Jariyawat
  • Thanapol Thammapratip
  • Kanoknetr Suksen
  • Podchanart Wanitchakool
  • Jintapat Nateewattana
  • Arthit Chairoungdua
  • Apichart Suksamrarn
  • Pawinee Piyachaturawat


Diarylheptanoids, isolated from the rhizome of Curcuma comosa Roxb., have several biological activities including anti-oxidant and anti-inflammation. The present study investigated the effect of five diarylheptanoids isolated from C. comosa rhizome on the proliferation of murine P388 leukemic cells. Compound-092, (3S)-1-(3,4-dihydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-ol, bearing a catechol moiety, was the most potent diarylheptanoid (IC50 of 4 μM) in inhibiting P388 leukemic cell viability by causing DNA breakage and inducing apoptosis. Apoptotic cell death was characterized by the presence of chromatin condensation, formation of apoptotic bodies, DNA fragmentation, and externalization of plasma membrane phosphatidylserine. This compound increased caspase-3 activity about fivefold above the untreated control, decreased the intracellular reduced glutathione level, and impaired mitochondrial transmembrane potential. In the presence of Cu(II) ion, the compound exhibited a pro-oxidant activity causing DNA strand breakage and enhancing the anti-proliferative activity. The results provide evidence for the pro-oxidant activity of the diarylheptanoid bearing a catechol moiety in the induction of apoptosis in murine P388 leukemia.


Apoptosis Catechol Leukemia Curcuma comosa Diarylheptanoid Pro-oxidant 



This study was supported by Mahidol University, Center of Excellence on Environmental Health, Toxicology and Management of Toxic Chemical, and the Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative and Thailand Research Fund (TRF). The authors thank Profs. Prapon Wilairat and Chumpol Pholpramool for their critical reading and comments on the manuscript.

Conflict of interest



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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Surawat Jariyawat
    • 1
  • Thanapol Thammapratip
    • 2
  • Kanoknetr Suksen
    • 1
  • Podchanart Wanitchakool
    • 2
  • Jintapat Nateewattana
    • 2
  • Arthit Chairoungdua
    • 1
  • Apichart Suksamrarn
    • 3
  • Pawinee Piyachaturawat
    • 1
    • 2
  1. 1.Department of Physiology, Faculty of ScienceMahidol UniversityBangkokThailand
  2. 2.Graduate Programme in Toxicology, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Department of Chemistry, Faculty of ScienceRamkhamhaeng UniversityBangkokThailand

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