Wide applications and extreme potential of metal oxide nanoparticles (NPs) increase occupational and public exposure and may yield extraordinary hazards for human health. Exposure to NPs has a risk for dysfunction of the vascular endothelial cells. The objective of this study was to assess the cytotoxicity of six metal oxide NPs to human cardiac microvascular endothelial cells (HCMECs) in vitro. Metal oxide NPs used in this study included zinc oxide (ZnO), iron(III) oxide (Fe2O3), iron(II,III) oxide (Fe3O4), magnesium oxide (MgO), aluminum oxide (Al2O3), and copper(II) oxide (CuO). The cell viability, membrane leakage of lactate dehydrogenase, intracellular reactive oxygen species, permeability of plasma membrane, and expression of inflammatory markers vascular cell adhesion molecule-1, intercellular adhesion molecule-1, macrophage cationic peptide-1, and interleukin-8 in HCMECs were assessed under controlled and exposed conditions (12–24 h and 0.001–100 μg/ml of exposure). The results indicated that Fe2O3, Fe3O4, and Al2O3 NPs did not have significant effects on cytotoxicity, permeability, and inflammation response in HCMECs at any of the concentrations tested. ZnO, CuO, and MgO NPs produced the cytotoxicity at the concentration-dependent and time-dependent manner, and elicited the permeability and inflammation response in HCMECs. These results demonstrated that cytotoxicity, permeability, and inflammation in vascular endothelial cells following exposure to metal oxide nanoparticles depended on particle composition, concentration, and exposure time.
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- Fe2O3 :
- Y2O3 :
- CeO2 :
- Fe3O4 :
- Al2O3 :
Endothelial cell medium
Human cardiac microvascular endothelial cells
Reactive oxygen species
Vascular cell adhesion molecule-1
Intercellular adhesion molecule 1
Macrophage cationic peptide-1
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This study was supported by grants from the Shanghai Municipal Health Bureau (2008Y077) and the Sub-Project of the National Grand Fundamental Research 863 Program of China (2007AA021802 and 2007AA022004).
Conflict of interest
The authors have no conflict of interest.
Jing Sun and Shaochuang Wang contributed equally in this study.
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Sun, J., Wang, S., Zhao, D. et al. Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells. Cell Biol Toxicol 27, 333–342 (2011). https://doi.org/10.1007/s10565-011-9191-9
- Metal oxide nanoparticles
- Vascular endothelial cells