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Catalysis Letters

, Volume 145, Issue 2, pp 519–526 | Cite as

Immobilization of Carbonic Anhydrase on Modified Electrospun Poly(Lactic Acid) Membranes: Quest for Optimum Biocatalytic Performance

  • Prakash C. Sahoo
  • Nonni Soraya Sambudi
  • Seung Bin Park
  • Jay H. Lee
  • Jong-In Han
Article

Abstract

Electrospun poly (lactic acid) membranes modified with graphene oxide (GO) and nano sized metal organic frameworks (nMOF) were synthesized, characterized and used for the immobilization of carbonic anhydrase (CA). The mechanical strength and young’s modulus of the membrane were slightly decreased when GO and nMOF were introduced, due to irregularity in the dispersion. On the other hand, the incorporation of GO into the membrane increased its thermal degradation temperature. Enzyme loading was substantially improved with GO and nMOF integration (~1.83 and ~1.57 times higher than that of poly (lactic acid)). Thermal stability, storage stability and reusability of immobilized CA, all of which are important for industrial applications, were also found to be enhanced significantly.

Graphical Abstract

Improved loading, stability and biocatalytic activity of carbonic anhydrase enzyme on modified electrospun membrane are reported

Keywords

Graphene oxide Nano-MOF Enzyme immobilization Electrospun PLA 

Notes

Acknowledgments

This research was supported financially by the Advanced Biomass R&D Center (ABC) (ABC-2012053875) Grant funded by the Ministry of Education, Science and Technology, Korea.

Supplementary material

10562_2014_1406_MOESM1_ESM.docx (664 kb)
Supplementary material 1 (DOCX 663 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea
  2. 2.Department of Civil and Environmental EngineeringKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea

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