Chorionic villi derived mesenchymal like stem cells and expression of embryonic stem cells markers during long-term culturing
- 305 Downloads
Mesenchymal stem cells (MSCs) can be obtained from a variety of human tissues. MSCs derived from placental chorionic villi of the first trimester are likely to resemble, biologically, embryonic stem cells (ESC), due to the earlier development stage of placenta. In the present study long-term cultures of MSC-like cells were assessed in order to evaluate MSCs multipotent characteristics and molecular features during the period of culture. CV-cells obtained from 10 samples of chorionic villus displayed typical fibroblastoid morphology, undergone 20 passages during a period of 120 days, maintaining a stable karyotype throughout long term expansion. The cells were positive, for CD90, CD73, CD105, CD29, CD44, HLA ABC antigens and negative for CD14, CD34, AC133, and HLA DR antigens as resulted from the flow cytometry analysis. CV-cells were differentiated in adipocytes, osteoblasts, chondrocytes and neuronal cells under specific culture conditions. The expression of the ESC-gene markers POU5F1 (Oct-4) and NANOG was observed at earliest stages (4–12 passages) and not at the late stages (14–20 passages) by RT-PCR analysis. ZFP42 and SOX2 expression were not detected. Moreover, CV-cells were found to express GATA4 but not NES (Nestin). Chorionic villi-derived cells possess multipotent properties, display high proliferation rate and self-renew capacity, share common surface antigens with adult MSCs and express certain embryonics stem cells gene markers. These characteristics highlight chorionic villi as an attractive source of MSCs for the needs of regenerative medicine.
KeywordsChorionic villi mesenchymal like cells (CV-MSCs) Adult mesenchymal stem cells (MSCs) Embryonic stem cells (ESCs) Long-term culturing Multipotency Pluripotency genes
E. Katsiani contribution to the paper was the conception, the design and the first drafting of the article. A. Garas and C. Skentou contribution was the acquisition of chorionic villi samples and participation in the analysis of data. A. Tsezou and K. Dafopoulos participated in the analysis and interpretation of data. CI Messini contributed to the drafting, A. Daponte contributed to the shortening of the revised manuscript and I.E. Messinis contributions were at the conception of study and the final approval.
Compliance with ethical standards
Conflict of interest
There are no conflicts of interests to be declared.
- Aluigi M, Foglii M, Curti A, Isidori A, Grupioni E, Chiodoni C, Colombo MP, Versura P, Grigioni AD, Ferri E et al (2006) Nucleofaction is an efficient non-viral transfection technique for bone marrow-derived mesenchymal stem cells. Stem Cells 24(2):454–461. doi: 10.1634/stemcells.2005-0198 PubMedCrossRefGoogle Scholar
- Alviano F, Fossati V, Marchionni C, Arpinati M, Bonsi L, Franchina M, Lanzoni G, Cantoni S, Cavallini C, Bianchi F et al (2007) Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro. BMC Dev Biol 21:7–11. doi: 10.1186/1471-213X-7-11 Google Scholar
- Avanzini MA, Bernardo ME, Cometa AM, Perotti C, Zaffaroni N, Novara F, Visai L, Moretta A, Del Fante C, Villa R, Ball LM (2009) Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood-and bone marrow-derived progenitors. Haematologica 94(12):1649–1660PubMedPubMedCentralCrossRefGoogle Scholar
- Bailo M, Soncini M, Vertua E, Signoroni PB, Sanzone S, Lombardi G, Arienti D, Calamani F, Zatti D, Paul P et al (2004) Engraftment potential of human amnion and chorion cells derived from term placenta. Transplantation 78(10):1439–1448. doi: 10.1097/01.TP.0000144606.84234.49 PubMedCrossRefGoogle Scholar
- Bossolasco P, Montemurro T, Cova L, Zangrossi S, Calzarossa C, Buiatiotis S, Soligo D, Bosari S, Silani V, Deliliers GL et al (2006) Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential. Cell Res 16(4):329–336. doi: 10.1038/sj.cr.7310043 PubMedCrossRefGoogle Scholar
- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini FC, Krause DS, Deans RJ, Keating A, Prockop DJ, Horwitz EM (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8(4):315–317. doi: 10.1080/14653240600855905 PubMedCrossRefGoogle Scholar
- Götherström C, West A, Liden J, Uzunel M, Lahesmaa R, Le Blanc K (2005) Difference in gene expression between human fetal liver and adult bone marrow mesenchymal stem cells. Haematologica 90(8):1017–1026Google Scholar
- Montzka K, Lassonczyk N, Tschöke B, Neuss S, Führmann T, Franzen R, Smeets R, Brook GA, Wöltje M (2009) Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression. BMC Neurosci 10:16. doi: 10.1186/1471-2202-10-16 PubMedPubMedCentralCrossRefGoogle Scholar
- Paul G, Özen I, Christophersen NS, Reinbothe T, Bengzon J, Visse E, Jansson K, Dannaeus K, Henriques-Oliveira C, Roybon L et al (2012) The adult human brain harbors multipotent perivascular mesenchymal stem cells. PLoS ONE 7(4):e35577. doi: 10.1371/journal.pone.0035577 PubMedPubMedCentralCrossRefGoogle Scholar
- Poloni A, Rosini V, Mondini E, Maurizi G, Mancini S, Discepoli G, Biasio S, Battaglini G, Berardinelli E, Serrani F, Leoni P (2008) Characterization and expansion of mesenchymal progenitor cells from first-trimester chorionic villi of human placenta. Cytotherapy 10(7):690–697. doi: 10.1080/14653240802419310 PubMedCrossRefGoogle Scholar
- Poloni A, Maurizi G, Babini L, Serrani F, Berardinelli E, Mancini S, Costantini B, Discepoli G, Leoni P (2011) Human mesenchymal stem cells from chorionic villi and amniotic fluid are not susceptible to transformation after extensive in vitro expansion. Cell Transplant 20(5):643–654. doi: 10.3727/096368910X536518 PubMedCrossRefGoogle Scholar
- Poloni A, Maurizi G, Serrani F, Mancini S, Discepoli G, Tranquilli AL, Bencivenga R, Leoni P (2012) Human AB serum for generation of mesenchymal stem cells from human chorionic villi: comparison with other source and other media including platelet lysate. Cell Prolif 45(1):66–75. doi: 10.1111/j.1365-2184.2011.00799.x PubMedCrossRefGoogle Scholar
- Roselli EA, Lazzati S, Iseppon F, Manganini M, Marcato L, Gariboldi MB, Maggi F, Grati FR, Simoni G (2013) Fetal mesenchymal stromal cells from cryopreserved human chorionic villi: cytogenetic and molecular analysis of genome stability in long-term cultures. Cytotherapy 15(11):1340–1351. doi: 10.1016/j.jcyt.2013.06.019 PubMedCrossRefGoogle Scholar
- Spitalieri P, Cortese G, Pietropolli A, Filareto A, Dolci S, Klinger FG, Giardina E, Di Cesare S, Bernardini L, Lauro D et al (2009) Identification of multipotent cytotrophoblast cells from human first trimester chorionic villi. Cloning Stem Cells 11(4):535–556. doi: 10.1089/clo.2009.0046 PubMedCrossRefGoogle Scholar
- Tondreau T, Meuleman N, Delforge A, Dejeneffe M, Leroy R, Massy M, Mortier C, Bron D, Lagneaux L (2005) Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity. Stem Cells 23(8):1105–1112. doi: 10.1634/stemcells.2004-0330 PubMedCrossRefGoogle Scholar
- Zheng C, Yang S, Guo Z, Liao W, Zhang L, Yang R, Han ZC (2009) Human multipotent mesenchymal stromal cells from fetal lung expressing pluripotent markers and differentiating into cell types of three germ layers. Cell Transplant 18(10):1093–1109. doi: 10.3727/096368909X12483162197042 PubMedCrossRefGoogle Scholar