Skin donors and human skin allografts: evaluation of an 11-year practice and discard in a referral tissue bank
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The Saint Louis hospital tissue bank provides skin allografts to pediatric and adult burn units in the Paris area. The aim of this study was to analyze our activity during the last 11 years focusing on the reasons for skin discard. Skin is procured solely from the back of the body, which is divided into 10 zones that are harvested and processed separately. This retrospective study included all skin donors harvested between June 2002 and June 2013, representing a total of 336 donors and 2770 zones. The donors were multiorgan heart-beating donors in 91 % of cases (n = 307). The main reason for discarding harvested skin was microbial contamination, detected in 99 donors (29 %). Most contaminants were of low pathogenicity. Other reasons for discard included positive serologic tests for 2 donors [17 zones (0.61 %)], unsuitable physical skin characteristics for 3 zones (0.11 %), the donor’s medical history for 53 zones (1.91 %), and technical issues with processing or distribution for 61 zones (2.2 %). In our experience, microbial contamination continues to be the main reason for discarding potential skin allografts. However, discards are limited by separate harvesting and processing of multiple zones in each donor.
KeywordsTissue banking Skin donors Skin allografts Discards Microbial contamination
Multi-organ heart-beating donors
The authors would like to thanks Dr. Jean-Luc Donay from the microbiology department of Saint Louis Hospital as well as Thierry Marchix, Chantal Schaffart, and all the members of the Tissue Bank technical team. Thanks to Dr. Sophie Olson for revising this manuscript.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest to declare.
- Agence de la biomédecine (2012). Annual reportGoogle Scholar
- Lannau B, Van Geyt C, Van Maele G, Beele H (2015) Analysis of potential factors affecting microbiological cultures in tissue donors during procurement. Cell Tissue Bank 16:65–71Google Scholar
- Neely AN, Plessinger RT, Stamper B, Kagan RJ (2008) Can contamination of a patient’s allograft be traced back to the allograft donor? J Burn Care Res: Off Publ Am Burn Assoc 29:73–76Google Scholar
- Pianigiani E, Risulo M, Ierardi F, Sbano P, Andreassi L, Fimiani M, Caudai C, Valensin PE, Zazzi M (2006) Prevalence of skin allograft discards as a result of serological and molecular microbiological screening in a regional skin bank in Italy. Burns J Int. Soc Burn Inj 32:348–351CrossRefGoogle Scholar
- Pirnay J-P, Verween G, Pascual B, Verbeken G, De Corte P, Rose T, Jennes S, Vanderkelen A, Marichal M, Heuninckx W et al (2012) Evaluation of a microbiological screening and acceptance procedure for cryopreserved skin allografts based on 14 day cultures. Cell Tissue Bank 13:287–295CrossRefPubMedPubMedCentralGoogle Scholar
- Verbeken G, Verween G, De Vos D, Pascual B, De Corte P, Richters C, De Coninck A, Roseeuw D, Ectors N, Rose T et al (2012) Glycerol treatment as recovery procedure for cryopreserved human skin allografts positive for bacteria and fungi. Cell Tissue Bank 13:1–7CrossRefPubMedPubMedCentralGoogle Scholar