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Cell and Tissue Banking

, Volume 16, Issue 4, pp 497–502 | Cite as

Effect of combination of nerve fragments with nerve growth factor in autologous epineurium small gap coaptation on peripheral nerve injury repair

  • Bo Feng
  • Hua Ma
  • He Hu
  • Lan Zhang
  • Zhi Zhang
  • Youming Pang
  • Yongjun Wang
  • Kecheng Niu
  • Ligong Lin
Article

Abstract

The aim of this study was to explore the effect of the combination of nerve fragments with nerve growth factor (NGF) on the repair of peripheral nerve injury through autologous epineurium small gap coaptation. A total of 150 male Sprague–Dawley rats weighing 200–250 g were divided into five groups randomly with 30 rats per group, including the following: a control group that was subjected to traditional end-to-end neuroanastomosis; an autologous epineurium small gap group that received autologous epineurium small gap coaptation suture; a nerve fragments group in which nerve fragments were added to the small gap; an NGF group in which NGF was added to the small gap; and an NGF combined with nerve fragments group in which both NGF and nerve fragments were added to the small gap. All groups were examined at 4, 6, and 8 weeks after the operation, respectively; furthermore, electroneurophysiological detection and histological observation were performed at 8 weeks. Autonomic activities and root ulcers recovered sooner in rats in the NGF combined with nerve fragments group than the other groups. Moreover, the numbers of regenerated nerve fibers were greater and nerve conduction velocity was faster in the NGF combined with nerve fragments group than the other groups. Therefore, the combination of NGF with nerve fragments plays a significant role in the repair of peripheral nerve injury through autologous epineurium small gap coaptation. Therefore, compared with the other four methods, the combination of nerve fragments with NGF on the repair of peripheral nerve injury through autologous epineurium small gap coaptation has a better effect.

Keywords

Nerve repair Small gap bridging Nerve debris Nerve growth factor 

Notes

Conflict of interest

All authors have no conflict of interest regarding this paper.

References

  1. Amado S, Rodrigues JM, Luís AL et al (2010) Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair. NeuroengRehabil 7:7Google Scholar
  2. Buti M, Verdu E, Labrador RO, Vilches JJ, Forés J, Navarro X (1996) Influence of physical parameters of nerve chambers on peripheral nerve regeneration and reinnervation. ExpNeurol 137:26–33Google Scholar
  3. Cajal RS (1928) Degeneration and regeneration of the nervous system. Oxford University Press, New YorkGoogle Scholar
  4. Chaiyasate K, Schafmer A, Jackson IT, Mittal V (2009) Comparing FK-506 with basic fibroblast growth factor (b-FGF) on the repair of a peripheral nerve defect using an autogenous vein bridge model. J Invest Surg 22:401–405CrossRefPubMedGoogle Scholar
  5. Daly W, Yao L, Zeugolis D, Windebank A, Pandit A (2012) A biomaterials approach to peripheral nerve regeneration: bridging the peripheral nerve gap and enhancing functional recovery. J R Soc Interface 9:202–221PubMedCentralCrossRefPubMedGoogle Scholar
  6. Frostick SP, Yin Q, Kemp GJ (1998) Schwann cells, neurotrophic factors, and peripheral nerve regeneration. Microsurgery 18:397–405CrossRefPubMedGoogle Scholar
  7. Jiang B, Zhang P, Zhang D, Fu Z, Yin X, Zhang H (2006) Study on small gap sleeve bridging peripheral nerve injury. Artif Cells Blood SubstitImmobilBioteehnol 34:55–74CrossRefGoogle Scholar
  8. Lu MC, Huang YT, Lin JH, Yao CH, Lou CW, Tsai CC, Chen YS (2009) Evaluation of a multi-layer microbraidedpolylactic acid fiber-reinforced conduit for peripheral nerve regeneration. J Mater Sci Mater Med 20:1175–1180CrossRefPubMedGoogle Scholar
  9. Napoli I, Noon LA, Ribeiro S et al (2012) A central role for the ERK-signaling pathway in controlling Schwann cell plasticity and peripheral nerve regeneration in vivo. Neuron 73:729–742CrossRefPubMedGoogle Scholar
  10. Nazario J, Kuffler DP (2011) Hyperbaric oxygen therapy and promoting neurological recovery following nerve trauma. Undersea Hyperb Med 38:345–366PubMedGoogle Scholar
  11. Okamoto H, Hata K, Kagami H et al (2010) Recovery process of sciatic nerve defect with novel bioabsorbable collagen tubes packed with collagen filaments in dogs. J Biomed Mater Res A 92:859–868PubMedGoogle Scholar
  12. Siemionow M, Bozkurt M, Zor F (2010) Regeneration and repair of peripheral nerves with different biomaterials: review. Microsurgery 30:574–588CrossRefPubMedGoogle Scholar
  13. Tang X, Xue C, Wang Y, Ding F, Yang Y, Gu X (2012) Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold. Biomaterials 33:3860–3867CrossRefPubMedGoogle Scholar
  14. Tos P, Battiston B, Ciclamini D, Geuna S, Artiaco S (2012) Primary repair of crush nerve injuries by means of biological tubulization with muscle-vein-combined grafts. Microsurgery 32:358–363CrossRefPubMedGoogle Scholar
  15. Tsujimoto H, Nakamura T, Miki T, Kubo T, Otsuji E, Yamagishi H, Hagiwara A (2011) Regeneration and functional recovery of intrapelvic nerves removed during extensive surgery by a new artificial nerve conduit: a breakthrough to radical operation for locally advanced and recurrent rectal cancers. J GastrointestSurg 15:1035–1042CrossRefGoogle Scholar
  16. Wang Y, Zhao Z, Ren Z et al (2012) Recellularized nerve allografts with differentiated mesenchymal stem cells promote peripheral nerve regeneration. NeurosciLett 514:96–101Google Scholar
  17. Wang Z, Zhang P, Kou Y, Yin X, Han N, Jiang B (2013) Hedysari extract improves regeneration after peripheral nerve injury by enhancing the amplification effect. PLoS One 8:e67921PubMedCentralCrossRefPubMedGoogle Scholar
  18. Whitlock EL, Tuffaha SH, Luciano JP et al (2009) Processed allografts and type I collagen conduits for repair of peripheral nerve gaps. Muscle Nerve 39:787–799CrossRefPubMedGoogle Scholar
  19. Yan Q, Yin Y, Li B (2012) Use new PLGL-RGD-NGF nerve conduits for promoting peripheral nerve regeneration. Biomed Eng Online 11:36PubMedCentralCrossRefPubMedGoogle Scholar
  20. Yang DY, Sheu ML, Su HL et al (2012) Dual regeneration of muscle and nerve by intravenous administration of human amniotic fluid-derived mesenchymal stem cells regulated by stromal cell-derived factor-1α in a sciatic nerve injury model. J Neurosurg 116:1357–1367CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Bo Feng
    • 1
  • Hua Ma
    • 1
  • He Hu
    • 1
  • Lan Zhang
    • 1
  • Zhi Zhang
    • 1
  • Youming Pang
    • 1
  • Yongjun Wang
    • 1
  • Kecheng Niu
    • 1
  • Ligong Lin
    • 1
  1. 1.Department of OsteologyThe Third Clinical Medical College of Inner Mongolia Medical UniversityBaotouChina

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