Advertisement

Cell and Tissue Banking

, Volume 16, Issue 2, pp 243–247 | Cite as

Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C

  • Mozhgan Rezaei KanaviEmail author
  • Mohammad Ali Javadi
  • Tahereh Chamani
  • Pejman Fahim
  • Fatemeh Javadi
Original Paper

Abstract

This study was conducted to compare the quantitative and qualitative indices of the donated corneas maintained in either Optisol-GS or Eusol-C storage media. In an ante-grade single blind study, two corneas from each donor with a death to preservation time of less than 30 h and with a minimum of “an apparent good cornea rating” were maintained in corneal storage media; randomly one in Optisol-GS and the other in Eusol-C. Slit-lamp biomicroscopic and specular microscopic examinations were performed on days 1 and 7. The results of the qualitative and quantitative indices and the final cornea rating were recorded. Statistical analyses were performed to evaluate any differences between the two media. 180 corneas from 90 donors with an age range of 29.3 ± 22.4 years were allocated in two groups: 90 corneas in Optisol-GS and the other 90 in Eusol-C. Five corneas in Optisol-GS and four corneas in Eusol-C were excluded from the study due to lack of specular images. There was no significant change of endothelial rating from day 1 to day 7 between two media (P = 0.175). As the maintenance time of the donated corneas increased, no significant difference was noted between the two groups in terms of endothelial indices, stromal edema, and Descemet’s folding. This study shows no superiority between Eusol-C and Optisol-GS in short term preservation of donated corneas.

Keywords

Optisol-GS Eusol-C Specular microscopy Cornea Endothelial indices 

Notes

Acknowledgments

The authors wish to gratefully acknowledge the assistance of Mrs. Mahnoosh Rezaei Kanavi, Mr. Hadi Kermanizadeh and Mr. Kambiz Bayat Makoo.

Conflict of interest

None.

References

  1. Bourdon E, Loreau N, Lagrost L, Blache D (2005) Differential effects of cysteine and methionine residues in the antioxidant activity of human serum albumin. Free Radic Res 39:15–20CrossRefPubMedGoogle Scholar
  2. Camposampiero D, Tiso R, Zanetti E, Ruzza A, Bruni A, Ponzin D (2003) Improvement of human corneal endothelium in culture after prolonged hypothermic storage. Eur J Ophthalmol 13(9–10):745–751PubMedGoogle Scholar
  3. Chaibpracob J et al (2006) New corneal preservation media. Eusol-C vs. Optisol-GS. Presented as abstract at EBAA 2006, Toronto, Canada Google Scholar
  4. Chan PS, Caron JP, Orth MW (2007) Effects of glucosamine and chondroitin sulfate on bovine cartilage explants under long-term culture conditions. Am J Vet Res 68:709–715CrossRefPubMedGoogle Scholar
  5. Darlington DN, Gann DS (2005) Purine nucleosides stimulate Na/K ATPase, and prolong survival in hemorrhagic shock. J Trauma 58:1055–1060CrossRefPubMedGoogle Scholar
  6. European Eye Bank Association (EEBA) Standards (2013) Technical Guidelines for Ocular Tissue, Revision 6. http://www.europeaneyebanks.org/files/Technical_Guidelines_Rev6_Feb2013.pdf
  7. Ferreira AG, Stefanello FM, Cunha AA, da Cunha MJ, Pereira TC, Bonan CD, Bogo MR, Netto CA, Wyse AT (2011) Role of antioxidants on Na(+), K (+)-ATPase activity and gene expression in cerebral cortex of hyperprolinemic rats. Metab Brain Dis 26:141–147CrossRefPubMedGoogle Scholar
  8. Jeng BH (2006) Preserving the cornea: corneal storage media. Curr Opin Ophthalmol 17:332–337CrossRefPubMedGoogle Scholar
  9. Medical Standards (2003) The Eye Bank Association of America Medical Standards Revision: A1.100 Scope, cyber-sight. http://www.cybersight.org/bins/content_page.asp?cid=1-1581-1775
  10. Mohammad A, Ali N, Reza B, Ali K (2012) Effect of ascorbic acid supplementation on nitric oxide metabolites and systolic blood pressure in rats exposed to lead. Indian J Pharmacol 42:78–81Google Scholar
  11. Rose GE (1986) Clinical assessment of corneal endothelial cell density: an original system of grading using a slit-lamp biomicroscope. Br J Ophthalmol 70:510–515CrossRefPubMedCentralPubMedGoogle Scholar
  12. Scott-Bryan G et al (2006) Eusol-C effectiveness as a corneal storage media. Presented at EEBA 2006, Venice, ItalyGoogle Scholar
  13. Spolarics Z, Lang CH, Bagby GJ, Spitzer JJ (1991) Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells. Am J Physiol 261:G185–G190PubMedGoogle Scholar
  14. Wilson SE, Bourne WM (1989) Corneal preservation. Surv Ophthalmol 33(4):237–259CrossRefPubMedGoogle Scholar
  15. Wu G, Greene LW (1992) Glutamine and glucose metabolism in bovine blood lymphocytes. Comp Biochem Physiol 103:821–825Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Mozhgan Rezaei Kanavi
    • 1
    • 2
    Email author
  • Mohammad Ali Javadi
    • 1
    • 2
  • Tahereh Chamani
    • 2
  • Pejman Fahim
    • 2
  • Fatemeh Javadi
    • 3
  1. 1.Ophthalmic Research CenterShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Central Eye Bank of IranTehranIran
  3. 3.Shahed University of Medical SciencesTehranIran

Personalised recommendations