Abstract
Prevalence of leg ulcer in general population is important and new efficient treatments are now needed, especially for chronic leg ulcers. Human amniotic membrane (HAM) can be used as an alternative treatment for recalcitrant leg ulcers. The aim of this study is to investigate the effects of a HAM extract on normal fibroblasts (NF) and ulcer fibroblasts (UF). NF and UF were obtained from biopsies by explants technique. HAM extract was used at 10 μg of total proteins per ml. Single patient-matched NF and UF were compared, without or with HAM extract. Studied parameters were proliferation rate, retraction of free-floating lattices, alpha smooth muscle actin expression by flow cytometry, and synthesis of elastin, glycosaminoglycans (GAGs), pro-collagen I, MMP-1 and TIMP-1. Our results show that UF had a specific phenotype compared to NF: low proliferation, high expression of alpha-SM actin and high synthesis of MMP-1, TIMP-1 and elastin. HAM extract significantly increased the synthesis of GAGs, pro-collagen I and MMP-1 in NF and decreased retraction of free lattices. HAM extract transiently increased UF proliferation, slowed down lattices retraction and decreased elastin synthesis. In conclusion, HAM extract has little effect on UF for the parameters studied and NF are more responsive than UF. However, clinical beneficial effect of HAM application on leg ulcers was previously observed and might rather be related to an action on keratinocytes and/or a modulation of the highly inflammatory environment of these chronic wounds.
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Acknowledgments
The authors would like to thank Oséo-Anvar for funding. The authors want to thank the AICT unit of the French Blood Establishment of Bourgogne Franche-Comté (EFS-BFC) for providing HAM. The authors finally want to thank Dr G. Rolin for helpful comments on this manuscript.
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H. Tauzin and S. Robin are co-authors of this study.
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Tauzin, H., Robin, S., Humbert, P. et al. Can leg ulcer fibroblasts phenotype be influenced by human amniotic membrane extract?. Cell Tissue Bank 15, 251–255 (2014). https://doi.org/10.1007/s10561-014-9422-4
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DOI: https://doi.org/10.1007/s10561-014-9422-4