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Cell and Tissue Banking

, Volume 15, Issue 2, pp 227–239 | Cite as

In toto differentiation of human amniotic membrane towards the Schwann cell lineage

  • Asmita Banerjee
  • Sylvia Nürnberger
  • Simone Hennerbichler
  • Sabrina Riedl
  • Christina M. A. P. Schuh
  • Ara Hacobian
  • Andreas Teuschl
  • Johann Eibl
  • Heinz Redl
  • Susanne Wolbank
Original Paper

Abstract

Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell properties. In its decellularized form it has been successfully applied as nerve conduit biomaterial to improve peripheral nerve regeneration in injury models. We hypothesize that viable hAM without prior cell isolation can be differentiated towards the Schwann cell lineage to generate a possible alternative to commonly applied tissue engineering materials for nerve regeneration. For in vitro Schwann cell differentiation, biopsies of hAM of 8 mm diameter were incubated with a sequential order of neuronal induction and growth factors for 21 days and characterized for cellular viability and the typical glial markers glial fibrillary acidic protein (GFAP), S100β, p75 and neurotrophic tyrosine kinase receptor (NTRK) using immunohistology. The secretion of the neurotrophic factors brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) was quantified by ELISA. The hAM maintained high viability, especially under differentiation conditions (90.2 % ± 41.6 day 14; 80.0 % ± 44.5 day 21 compared to day 0). Both, BDNF and GDNF secretion was up-regulated upon differentiation. The fresh membrane stained positive for GFAP and p75 and NTRK, which was strongly increased after culture in differentiation conditions. Especially the epithelial layer within the membrane exhibited a change in morphology upon differentiation forming a multi-layered epithelium with intense accumulations of the marker proteins. However, S100β was expressed at equal levels and equal distribution in fresh and cultured hAM conditions. Viable hAM may be a promising alternative to present formulations used for peripheral nerve regeneration.

Keywords

Schwann cells Human amniotic membrane In toto differentiation Amniotic epithelial cells Peripheral nerve regeneration Adipose-derived stem cells 

Notes

Acknowledgments

We would like to thank Daniela Dopler, Alice Zimmermann and Sidrah Chaudry for their technical assistance and Eva Schwingenschlögl for support with graphic design.

Conflict of interest

We would like to disclose that the co-authors Johann Eibl and Heinz Redl own the patent rights for “Process for differentiating stem cells of the amniotic membrane”.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Asmita Banerjee
    • 1
    • 2
  • Sylvia Nürnberger
    • 1
    • 7
    • 8
  • Simone Hennerbichler
    • 2
    • 3
  • Sabrina Riedl
    • 1
    • 2
  • Christina M. A. P. Schuh
    • 1
    • 2
  • Ara Hacobian
    • 1
    • 2
  • Andreas Teuschl
    • 2
    • 5
    • 6
  • Johann Eibl
    • 4
  • Heinz Redl
    • 1
    • 2
  • Susanne Wolbank
    • 1
    • 2
  1. 1.Ludwig Boltzmann Institute for Experimental and Clinical TraumatologyAUVA Research CenterViennaAustria
  2. 2.Austrian Cluster for Tissue RegenerationViennaAustria
  3. 3.Red Cross Blood Transfusion Service for Upper AustriaLinzAustria
  4. 4.Bio-Products and Bio-Engineering AGViennaAustria
  5. 5.City of Vienna Competence Team Tissue Engineering BioreactorsUniversity of Applied Science Technikum WienViennaAustria
  6. 6.Department of Biochemical EngineeringUniversity of Applied Science Technikum WienViennaAustria
  7. 7.Department of TraumatologyMedical University of ViennaViennaAustria
  8. 8.Bernhard Gottlieb University Clinic of DentistryViennaAustria

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