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Cell and Tissue Banking

, Volume 15, Issue 1, pp 127–137 | Cite as

Wrapped omentum with periosteum concurrent with adipose derived adult stem cells for bone tissue engineering in dog model

  • Amin Bigham Sadegh
  • Ehsan Basiri
  • Ahmad Oryan
  • Pezhman Mirshokraei
Original Paper

Abstract

Adipose derived adult stem cells (ASCs) are multipotent cells that are able to differentiate into osteoblasts in presence of certain factors. The histological characteristics of periosteum makes it a specific tissue with a unique capacity to be engineered. Higher flexibility of the greater omentum is useful for reconstructive surgery. These criteria make it suitable for tissue engineering. The present study was designed to evaluate bone tissue engineering with periosteal free graft concurrent with ASCs and pedicle omentum in dog model. Twelve young female indigenous dogs were used in this experiment. In omental group (n = 4), end of omentum was wrapped by periosteum of the radial bone in abdomen of each dog. In omental-autogenously ASCs group (n = 4), 1 ml of ASCs was injected into the wrapped omentum with periosteum while in omental-allogenously ASCs group (n = 4), 1 ml of allogenous ASCs was injected. Lateral view radiographs were taken from the abdominal cavity postoperatively at the 2nd, 4th, 6th and 8th weeks post-surgery. Eight weeks after operation the dogs were re-anesthetized and the wrapped omenum by periosteum in all groups was found and removed for histopathological evaluation. Our results showed that omentum–periosteum, omental-periosteum-autogenous ASCs and omental-periosteum-allogenous ASCs groups demonstrated bone tissue formation in the abdominal cavity in dog model. The radiological, macroscopical and histological findings of the present study by the end of 8 weeks post-surgery indicate bone tissue engineering in all three groups in an equal level. The present study has shown that the wrapped omentum with periosteum concurrent with ASCs (autogenous or allogenous ASCs) lead to a favorable bone tissue formation. We suggested that it may be useful when pedicle graft omentum used concurrent with periosteum in the bone defect reconstruction, and this phenomenon should be studied in future.

Keywords

ASCs Omentum Periosteum Bone tissue engineering 

Notes

Acknowledgments

The authors would like to thank the Iranian National Science Foundation for their financial support and cooperation.

Conflict of interest

All authors declare that there is no conflict of interests.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Amin Bigham Sadegh
    • 1
  • Ehsan Basiri
    • 1
  • Ahmad Oryan
    • 2
  • Pezhman Mirshokraei
    • 3
    • 4
  1. 1.Department of Veterinary Surgery and Radiology, School of Veterinary MedicineShahrekord UniversityShahrekordIran
  2. 2.Department of Veterinary Pathology, School of Veterinary MedicineShiraz UniversityShirazIran
  3. 3.Research Institute of Animal Embryo TechnologyShahrekord UniversityShahrekordIran
  4. 4.Department of Clinical Sciences, School of Veterinary MedicineFerdowsi University of MashhadMashhadIran

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