Cell and Tissue Banking

, Volume 14, Issue 3, pp 401–406 | Cite as

Bacterial contamination of amniotic membrane in a tissue bank from Iran

  • Hamid Reza Aghayan
  • Parisa Goodarzi
  • Alireza Baradaran-Rafii
  • Bagher Larijani
  • Leila Moradabadi
  • Fakher Rahim
  • Babak Arjmand
Original Paper


Human Amniotic Membrane (AM) transplantation can promote tissue healing and reduce inflammation, tissue scarring and neovascularization. Homa Peyvand Tamin (HPT) tissue bank has focused on manufacturing human cell and tissue based products including AM. The purpose of this study is to evaluate and identify bacterial contamination of AMs that is produced by HPT for several ophthalmic applications. From July 2006 to April 2011, 122 placentas from cesarean sections were retrieved by HPT after obtaining informed consent from the donors. Besides testing donor’s blood sample for viral markers, microbiological evaluation was performed pre and post processing. During tissue processing, decontamination was performed by an antibiotic cocktail including; Gentamicin, Ceftriaxone and Cloxacillin. Of 271 cesarean section AM donors who were screened as potential donors, 122 were accepted for processing and assessed for microbiological contamination. Donors’ age were between 21 and 41 years (Mean = 27.61 ± 0.24). More than 92 % of mothers were in their first or second gravidity with full term pregnancies. The most prevalent organisms were Staphylococci species (72.53 %). After processing, contamination rates markedly decreased by 84.62 % (p value = 0.013). According to our results, most of bacterial contaminations were related to donation process and the contamination pattern suggests procurement team as a source. Therefore we recommend that regular training programs should be implemented by tissue banks for procurement staff. These programs should focus on improved donor screening and proper aseptic technique for tissue retrieval. We also suggest that tissue banks should periodically check the rate and types of tissue contaminations. These data help them to find system faults and to update processing methods.


Amniotic membrane Bacterial contamination Tissue banking 



The authors would like to acknowledge Dr. Seyed Majid Manavi, Dr. Farhad Zargari, Dr. Bahram Moazami, Maryam-Sadat Gousheh, Mehrnaz SahebJam, Firoozeh Ghaderi, and Maryam Kavousi.


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Hamid Reza Aghayan
    • 1
  • Parisa Goodarzi
    • 2
  • Alireza Baradaran-Rafii
    • 3
  • Bagher Larijani
    • 4
  • Leila Moradabadi
    • 5
  • Fakher Rahim
    • 6
  • Babak Arjmand
    • 1
    • 2
  1. 1.Endocrinology and Metabolism Research Center & Cellul Fanavaran Science-Based CompanyTehran University of Medical SciencesTehranIran
  2. 2.Brain and Spinal Injury Research CenterTehran University of Medical Sciences & Homa Peyvand Tamin (HPT) Tissue BankTehranIran
  3. 3.Ophthalmic Research Center, Labbafi-nejad Medical CenterShahid Behashti University of Medical SciencesTehranIran
  4. 4.Endocrinology and Metabolism Research CenterTehran University of Medical SciencesTehranIran
  5. 5.Cell Manufacturing Facility, Novin Darman BorhanTehranIran
  6. 6.Toxicology Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran

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