Osteoconductivity and osteoinductivity of NanoFUSE® DBM
- 298 Downloads
Bone graft substitutes have become an essential component in a number of orthopedic applications. Autologous bone has long been the gold standard for bone void fillers. However, the limited supply and morbidity associated with using autologous graft material has led to the development of many different bone graft substitutes. Allogeneic demineralized bone matrix (DBM) has been used extensively to supplement autograft bone because of its inherent osteoconductive and osteoinductive properties. Synthetic and natural bone graft substitutes that do not contain growth factors are considered to be osteoconductive only. Bioactive glass has been shown to facilitate graft containment at the operative site as well as activate cellular osteogenesis. In the present study, we present the results of a comprehensive in vitro and in vivo characterization of a combination of allogeneic human bone and bioactive glass bone void filler, NanoFUSE® DBM. NanoFUSE® DBM is shown to be biocompatible in a number of different assays and has been cleared by the FDA for use in bone filling indications. Data are presented showing the ability of the material to support cell attachment and proliferation on the material thereby demonstrating the osteoconductive nature of the material. NanoFUSE® DBM was also shown to be osteoinductive in the mouse thigh muscle model. These data demonstrate that the DBM and bioactive glass combination, NanoFUSE® DBM, could be an effective bone graft substitute.
KeywordsDemineralized bone matrix Bioactive glass Osteoconductivity Osteoinductivity
The authors would like to thank the donors and their families for their selfless gift of tissue donation, without which this research would not have been possible. The authors would also like to thank Nanotherapeutics, Inc. for their continued support of this research.
- Hattar S, Berdal A, Asselin A, Loty S, Greenspan DC, Sautier JM (2002) Behaviour of moderately differentiated osteoblast-like cells cultured in contact with bioactive glasses. Eur Cells Mater 4:61–69Google Scholar
- Mauney JR, Sjostorm S, Blumberg J, Horan R, O’Leary JP, Vunjak-Novakovic G, Volloch V, Kaplan DL (2004b) Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. Calcif Tissue Int 74(5):458–468. doi: 10.1007/s00223-003-0104-7 PubMedCrossRefGoogle Scholar
- Wheeler DL, Stokes KE, Hoellrich RG, Chamberland DL, McLoughlin SW (1998) Effect of bioactive glass particle size on osseous regeneration of cancellous defects. J Biomed Mater Res 41(4):527–533. doi: 10.1002/(SICI)1097-4636(19980915)41:4<527:AID-JBM3>3.0.CO;2-E PubMedCrossRefGoogle Scholar
- Xynos ID, Edgar AJ, Buttery LD, Hench LL, Polak JM (2000a) Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochem Biophys Res Commun 276(2):461–465. doi: 10.1006/bbrc.2000.3503 PubMedCrossRefGoogle Scholar
- Xynos ID, Hukkanen MV, Batten JJ, Buttery LD, Hench LL, Polak JM (2000b) Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation In vitro: implications and applications for bone tissue engineering. Calcif Tissue Int 67(4):321–329. doi: 10.1007/s002230001134 PubMedCrossRefGoogle Scholar
- Xynos ID, Edgar AJ, Buttery LD, Hench LL, Polak JM (2001) Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. J Biomed Mater Res 55(2):151–157. doi: 10.1002/1097-4636(200105)55:2<151:AID-JBM1001>3.0.CO;2-D PubMedCrossRefGoogle Scholar