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Cell and Tissue Banking

, Volume 6, Issue 4, pp 277–285 | Cite as

Processing of Whole Femoral Head Allografts: Validation Methodology for the Reliable Removal of Nucleated Cells, Lipid and Soluble Proteins Using a Multi-step Washing Procedure

  • P. Yates
  • J. Thomson
  • G. Galea
Article

Abstract

Following the major outbreak of BSE in the United Kingdom in the mid 1980's and the first reported cases of the human disease equivalent, vCJD, in 1996 a number of measures were taken to reduce the risk of secondary transmission of vCJD by blood or surgical instruments. Two cases of probable transmission of vCJD by blood components have now been reported. The Department of Health has recently undertaken a vCJD risk analysis for bone. Although significant uncertainties still exist in the assumptions on which the assessment is based, a few important recommendations were made. These include a recommendation that bone should be processed to remove blood and marrow and that pooling of donations is never advantageous. This study aimed to establish a method of processing whole femoral heads using disposable equipment, with the purpose of removing as much of the marrow components as possible. We are able to remove 98.2% (range 94.5–99.9%) of the nucleated cells and 98.7% (range 97.8–99.4%) of the soluble protein content from within an intact femoral head. This work confirms that the majority of blood and marrow components can be removed without the need for morselising the bone prior to washing.

Keywords

Allograft Bone Bone marrow components Femoral heads Processing 

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References

  1. Centres for Disease Control1988Transmission of HIV through bone transplantation: Case report and public health recommendationsMMWR37597599Google Scholar
  2. Conrad, E.U., Gretch, D., Obermeyer, K., Moogk, M., Sayers, M., Wilson, J., Strong, D.M. 1995The transmission of hepatitis C virus by tissue transplantationJ. Bone Joint Surg.77A214224Google Scholar
  3. Davies A.W. and Taylor K. 1965. Application of the auto analyser in a river authority laboratory. Presented at the Technicon Fifth International Symposium `Automation in Analytical Chemistry', October 1965, London, pp. 294–300.Google Scholar
  4. Eggen, B.M., Nordbo, S.A. 1992Transmission of HCV by organ transplantationN. Engl. J. Med.326411PubMedGoogle Scholar
  5. Galea, G., Kearney, J.N. 2005Clinical effectiveness of processed and unprocessed boneTransf. Med.15165174Google Scholar
  6. Health Services Circular `HSC1999/178 Variant Creutzfeldt-Jakob Disease (vCJD)' Department of Health London.Google Scholar
  7. Health Services Circular `HSC 1999/179 Controls assurance in infection control: decontamination of medical devices', Department of Health London.Google Scholar
  8. Health Services Circular `HSC200/032 Decontamination of medical devices' Department of Health London.Google Scholar
  9. Llewelyn, C.A., Hewitt, P.E., Knight, R.S.G., Amar, K., Cousens, S., Mackenzie, J., Will, R.G. 2004Possible transmission of variant Creutzfedt-Jacob disease by blood transfusionLancet363417421CrossRefPubMedGoogle Scholar
  10. Lomas, R., Drummond, O., Kearney, J.N. 2000Processing of whole femoral head allografts: A method for improving clinical efficacy and safetyCell Tissue Banking1193200CrossRefGoogle Scholar
  11. Lowry, O.H., Rosebrough, N.J., Farr, L., Randall, R.J. 1951Protein measurement with the Folin Phenol ReagentJ. Biol. Chem.193267275Google Scholar
  12. Peden, A.H., Head, M.W., Ritchie, D.L., Bell, J.E., Ironside, J.W. 2004Preclinical vCJD after blood transfusion in a PRNP codon 129 heterozygous patientLancet364527529CrossRefPubMedGoogle Scholar
  13. Pruss, A., Kao, M., von Garrel, T., Frommelt, L., Gurtler, L., Benedix, F., Pauli, G.,  et al. 2003aVirus inactivation in bone tissue transplants (femoral heads) by moist heat with the Marburg bone bank systemBiologicals317582Google Scholar
  14. Pruss, A., Seibold, M., Benedix, F., Frommelt, L., Garrel, T., Gurtler, L., Dorffel, Y., Pauli, G., Gobel, UB.,  et al. 2003bValidation of the `Marburg bone bank system' for thermodisinfection of allogeneic femoral head transplants using selected bacteriafungi and sporesBiologicals31287294Google Scholar
  15. Sanzen, L., Carlson, A. 1997Transmission of human T-cell lymphotrophic virus-type I by a deep-frozen bone allograftActa. Orthop.Scand687274PubMedGoogle Scholar
  16. Simonds, R.J., Holmberg, S.D., Hurwitz, R.L., Coleman, T.R., Bottenfield, S., Conley, L.J., Kohlenberg, S.H., Castro, K.G., Dahan, B.A., Schable, C.A., Rayfield, M.A., Rogers, M.F. 1992Transmission of human immunodeficiency virus type 1 from a seronegative organ and tissue donorN Engl J Med326726732PubMedCrossRefGoogle Scholar
  17. Singer, V.L., Jones, L.J., Yue, S.T., Haugland, R.P. 1997Characterisation of PicoGreen reagent and development of fluorescence-based solution assay for double-stranded DNA quantitationAnal. Biochem.249228238CrossRefPubMedGoogle Scholar
  18. The Feeding Stuffs (Sampling and Analysis) Regulations (as amended) Statutory Instrument 1985. No. 1119, HMSO, London BS4401 PART 4:1970.Google Scholar
  19. Will, R.G., Ironside, J.W., Zeidler, M., Cousens, S.N., Estibeiro, K., Alperovitch, A., Pose, S., Pocchiari, M., Hoffman, A., Smith, P.G. 1996A new variant of Creutzfeldt-Jakob disease in the UKLancet347921925CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Tissue Service DirectorateScottish National Blood Transfusion ServiceScotland
  2. 2.Regional Transfusion CentreAberdeenScotland

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