Abstract
Following the major outbreak of BSE in the United Kingdom in the mid 1980's and the first reported cases of the human disease equivalent, vCJD, in 1996 a number of measures were taken to reduce the risk of secondary transmission of vCJD by blood or surgical instruments. Two cases of probable transmission of vCJD by blood components have now been reported. The Department of Health has recently undertaken a vCJD risk analysis for bone. Although significant uncertainties still exist in the assumptions on which the assessment is based, a few important recommendations were made. These include a recommendation that bone should be processed to remove blood and marrow and that pooling of donations is never advantageous. This study aimed to establish a method of processing whole femoral heads using disposable equipment, with the purpose of removing as much of the marrow components as possible. We are able to remove 98.2% (range 94.5–99.9%) of the nucleated cells and 98.7% (range 97.8–99.4%) of the soluble protein content from within an intact femoral head. This work confirms that the majority of blood and marrow components can be removed without the need for morselising the bone prior to washing.
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InstitutionalAuthorNameCentres for Disease Control (1988) ArticleTitleTransmission of HIV through bone transplantation: Case report and public health recommendations MMWR 37 597–599
E.U. Conrad D. Gretch K. Obermeyer M. Moogk M. Sayers J. Wilson D.M. Strong (1995) ArticleTitleThe transmission of hepatitis C virus by tissue transplantation J. Bone Joint Surg. 77A 214–224
Davies A.W. and Taylor K. 1965. Application of the auto analyser in a river authority laboratory. Presented at the Technicon Fifth International Symposium `Automation in Analytical Chemistry', October 1965, London, pp. 294–300.
B.M. Eggen S.A. Nordbo (1992) ArticleTitleTransmission of HCV by organ transplantation N. Engl. J. Med. 326 411 Occurrence Handle1309597 Occurrence Handle1:STN:280:DyaK387gslansA%3D%3D
G. Galea J.N. Kearney (2005) ArticleTitleClinical effectiveness of processed and unprocessed bone Transf. Med. 15 IssueID3 165–174 Occurrence Handle1:STN:280:DC%2BD2M3pvVyktg%3D%3D
Health Services Circular `HSC1999/178 Variant Creutzfeldt-Jakob Disease (vCJD)' Department of Health London.
Health Services Circular `HSC 1999/179 Controls assurance in infection control: decontamination of medical devices', Department of Health London.
Health Services Circular `HSC200/032 Decontamination of medical devices' Department of Health London.
C.A. Llewelyn P.E. Hewitt R.S.G. Knight K. Amar S. Cousens J. Mackenzie R.G. Will (2004) ArticleTitlePossible transmission of variant Creutzfedt-Jacob disease by blood transfusion Lancet 363 417–421 Occurrence Handle10.1016/S0140-6736(04)15486-X Occurrence Handle14962520 Occurrence Handle1:STN:280:DC%2BD2c%2FnsVWhuw%3D%3D
R. Lomas O. Drummond J.N. Kearney (2000) ArticleTitleProcessing of whole femoral head allografts: A method for improving clinical efficacy and safety Cell Tissue Banking 1 193–200 Occurrence Handle10.1023/A:1026512312385 Occurrence Handle1:STN:280:DC%2BD2czltlSmsQ%3D%3D
O.H. Lowry N.J. Rosebrough L. Farr R.J. Randall (1951) ArticleTitleProtein measurement with the Folin Phenol Reagent J. Biol. Chem. 193 267–275
A.H. Peden M.W. Head D.L. Ritchie J.E. Bell J.W. Ironside (2004) ArticleTitlePreclinical vCJD after blood transfusion in a PRNP codon 129 heterozygous patient Lancet 364 527–529 Occurrence Handle10.1016/S0140-6736(04)16811-6 Occurrence Handle15302196
A. Pruss M. Kao T. von Garrel L. Frommelt L. Gurtler F. Benedix G. Pauli et al. (2003a) ArticleTitleVirus inactivation in bone tissue transplants (femoral heads) by moist heat with the Marburg bone bank system Biologicals 31 75–82
A. Pruss M. Seibold F. Benedix L. Frommelt T. Garrel Particlevon L. Gurtler Y. Dorffel G. Pauli UB. Gobel et al. (2003b) ArticleTitleValidation of the `Marburg bone bank system' for thermodisinfection of allogeneic femoral head transplants using selected bacteriafungi and spores Biologicals 31 287–294
L. Sanzen A. Carlson (1997) ArticleTitleTransmission of human T-cell lymphotrophic virus-type I by a deep-frozen bone allograft Acta. Orthop.Scand 68 72–74 Occurrence Handle9057574 Occurrence Handle1:STN:280:DyaK2s3htVGjsw%3D%3D
R.J. Simonds S.D. Holmberg R.L. Hurwitz T.R. Coleman S. Bottenfield L.J. Conley S.H. Kohlenberg K.G. Castro B.A. Dahan C.A. Schable M.A. Rayfield M.F. Rogers (1992) ArticleTitleTransmission of human immunodeficiency virus type 1 from a seronegative organ and tissue donor N Engl J Med 326 726–732 Occurrence Handle1738377 Occurrence Handle1:STN:280:DyaK387kvFKitQ%3D%3D Occurrence Handle10.1056/NEJM199203123261102
V.L. Singer L.J. Jones S.T. Yue R.P. Haugland (1997) ArticleTitleCharacterisation of PicoGreen reagent and development of fluorescence-based solution assay for double-stranded DNA quantitation Anal. Biochem. 249 IssueID2 228–238 Occurrence Handle10.1006/abio.1997.2177 Occurrence Handle9212875 Occurrence Handle1:CAS:528:DyaK2sXktF2rsbo%3D
The Feeding Stuffs (Sampling and Analysis) Regulations (as amended) Statutory Instrument 1985. No. 1119, HMSO, London BS4401 PART 4:1970.
R.G. Will J.W. Ironside M. Zeidler S.N. Cousens K. Estibeiro A. Alperovitch S. Pose M. Pocchiari A. Hoffman P.G. Smith (1996) ArticleTitleA new variant of Creutzfeldt-Jakob disease in the UK Lancet 347 921–925 Occurrence Handle10.1016/S0140-6736(96)91412-9 Occurrence Handle8598754 Occurrence Handle1:STN:280:DyaK287nvVKruw%3D%3D
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Yates, P., Thomson, J. & Galea, G. Processing of Whole Femoral Head Allografts: Validation Methodology for the Reliable Removal of Nucleated Cells, Lipid and Soluble Proteins Using a Multi-step Washing Procedure. Cell Tissue Banking 6, 277–285 (2005). https://doi.org/10.1007/s10561-005-1235-z
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DOI: https://doi.org/10.1007/s10561-005-1235-z