Detection of living cells in non-processed but deep-frozen bone allografts
- 143 Downloads
Impacted morselized donor bone is successfully used to treat bone loss in revision total hip arthroplasties. It is generally thought, but not proven, that the processing and storage at −80 °C of the donor bone kills all cells. Because of the risk of contamination and to increase our understanding about the process of new bone formation after revision total hip arthroplasty, the aim of this study was to investigate whether the donor bone does contain vital cells. Samples from 11 femoral heads were obtained according to the American and European standards of bone banking, and tested for their capacity to give rise to proliferating cells, using tissue culture methods. All bone samples were stored at − 80°C for a minimum of 6 months. Bone sample cores were morselized and cultured for 6 weeks. Inverted phase contrast microscopy was used to evaluate cell growth. DNA marker analysis was used to confirm celluar identity.
All bank bone samples gave rise to cell growth. The cell cultures showed osteoblastic characteristics in that they expressed high levels of alkaline phosphatase activity. DNA marker analysis showed identical alleles for cultured cells from frozen bone and freshly obtained buccal cells from the same donor, indicating that the cells growing from the banked bone were indeed originating from the donor tissue. It was therefore concluded that −80 °C freezing of bone tissue does not routinely kill cells within the tissue.
KeywordsBone bank Bone cell Femoral head allograft Impaction grafting Total hip arthroplasty
Unable to display preview. Download preview PDF.
- American Association of Tissue Banks 1996 Standards for Tissue Banking [editorial] American Association of Tissue BanksGoogle Scholar
- Aubin, J.E., Liu, F. 1996The osteoblast lineageBilezikian, J.P.Raisz, L.G.Rodan, G.A. eds. Principles of Bone BiologyAcademic PressSan DiegoCA5168Google Scholar
- Beresford, J.N., Gallagher, J.A., Gowen, M., McGuire, M.K.B., Poser, J., Russell, R.G.G. 1983Human bone cells in culture. A novel system for the investigation of bone cell metabolismClin. Sci. (Colch)643839Google Scholar
- Biezen van, F.C., ten Have, B.L., Verhaar, J.A. 2000Impaction bone-grafting of severely defective femora in revision total hip surgery: 21 hips followed for 41–85 monthsActa Orthop. Scand.71135142Google Scholar
- Burwell, R.G., Gowland, G. 1962aStudies in the transplantation of Bone III. The immune responses of lymph nodes draining components of fresh homologous cancellous bone and homologous bone treated by different methodsJ. Bone Joint Surg.44-B131Google Scholar
- Burwell, R.G. 1962bStudies in the transplantation of bone IV. The immune response of lymph nodes draining second-set homografts of fresh cancellous boneJ. Bone Joint Surg.44-B688Google Scholar
- Centers for Disease Control.1988Transmission of HIV through bone transplantation: case report and public health recommendationsMMWR Morb. Mortal. Wkly. Rep.37597599Google Scholar
- Centers for Disease Control.2003Hepatitis C virus transmissions from an antibody-negative organ and tissue donor – United States, 2002–2002MMWR Morb. Mortal. Wkly. Rep.52273276Google Scholar
- European Association for Musculo Skeletal Transplantation. 1997 Common Standards for Musculoskeletal Tissue Banking [editorial] European Association for Musculo Skeletal TransplantationGoogle Scholar
- Goldberg, V.M., Stevenson, S. 1987Natural history of autografts and allograftsClin. Orthop.␣716Google Scholar
- Mankin, H.J., Friedlaender, G.E. 1989Bone and Cartilage Allografts: Physiological and Immunological PrinciplesStock-IncThorofare(NJ)Bone Stock Deficiency in Total Hip Replacement.Google Scholar
- Schimmel, J.W. 1995Acetabular Reconstruction with Impacted Morsellized Cancellous Bone Grafts in Cemented Revision Hip ArthroplastyNijmegen UniversityNijmegen (The Netherlands)Google Scholar
- T.J. Slooff P. Buma B.W. Schreurs J.W. Schimmel R. Huiskes J. Gardeniers 1996 Acetabular and femoral reconstruction with impacted graft and cement Clin. Orthop. 108 Google Scholar
- Tagil, M., Aspenberg, P. 1998Impaction of cancellous bone grafts impairs osteoconduction in titanium chambersClin. Orthop.␣231238Google Scholar