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Cardiovascular Drugs and Therapy

, Volume 24, Issue 2, pp 121–130 | Cite as

Granulocyte-colony Stimulating Factor Treatment of Chronic Myocardial Infarction

  • Ruy A. N. Louzada
  • Patricia F. Oliveira
  • Joao Paulo A. Cavalcanti-de-Albuquerque
  • Leandro Cunha-Carvalho
  • Marcelo R. Baldanza
  • Taís H. Kasai-Brunswick
  • Regina C. S. Goldenberg
  • Antonio C. Campos-de-Carvalho
  • Joao P. S. Werneck-de-Castro
Article

Abstract

Purpose

The aim of this study was to investigate the impact of granulocyte-colony stimulating factor (G-CSF) administration on cardiac function of rats with chronic myocardial infarction through two different protocols: high dose short term and low dose long term protocols.

Methods

Wistar rats were submitted to MI surgery and after 4 weeks they received recombinant human G-CSF (Filgrastim) or vehicle subcutaneously. We tested the classical protocol (50 µg/kg/day during 7 days) and the long term low dose treatment (four cycles of 5 days of 10 µg/kg/day). Cardiac performance was evaluated before, 4 and 6 weeks after G-CSF injections by electro- and echocardiography, hemodynamic and treadmill exercise test.

Results

All infarcted groups exhibited impaired function compared to sham operated animals. Moreover, all cardiac functional parameter were not different between G-CSF and Vehicle group at resting conditions as well as after treadmill exercise stress test, despite intense white blood cell mobilization in both protocols at all time points. Hypertrophy was not different and infarct size was similar in histological analysis

Conclusions

These data clearly show that G-CSF treatment was unable to restore cardiac function impaired by myocardial infarction either with classical approach or long term low dose administration.

Key words

Stem cell mobilization Myocardial infarction Exercise test and cell therapy 

Notes

Funding

The present work was supported by CNPq, CAPES and FAPERJ.

Conflict of interest

All authors have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ruy A. N. Louzada
    • 1
    • 2
  • Patricia F. Oliveira
    • 1
    • 3
  • Joao Paulo A. Cavalcanti-de-Albuquerque
    • 2
  • Leandro Cunha-Carvalho
    • 2
  • Marcelo R. Baldanza
    • 2
  • Taís H. Kasai-Brunswick
    • 1
  • Regina C. S. Goldenberg
    • 1
  • Antonio C. Campos-de-Carvalho
    • 1
    • 4
  • Joao P. S. Werneck-de-Castro
    • 1
    • 2
    • 5
  1. 1.Laboratório de Cardiologia Celular e Molecular do Instituto de Biofísica Carlos Chagas FilhoUFRJRio de JaneiroBrasil
  2. 2.Laboratório de Biologia do Exercício do Departamento de Biociências e Atividade FísicaEEFD—UFRJRio de JaneiroBrasil
  3. 3.Área de Ciências Fisiológicas, Instituto de Ciências BiomédicasUniversidade Federal de UberlândiaUberlândiaBrasil
  4. 4.Albert Einstein College of MedicineBronxUSA
  5. 5.Departamento de Biociências e Atividade Física, CCS, Escola de Educação Física e Desportos, Ilha do FundãoUniversidade Federal do Rio de Janeiro (UFRJ)Rio de JaneiroBrasil

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